THE™ V5 Tag Antibody, mAb, Mouse

Art No | A01724

THE™ V5 Tag Antibody, mAb, Mouse

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Description

THE™ V5 Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.

Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA - recommended usage: 0.05-0.1 µg/ml
Western Blot - recommended usage: 0.2-1 µg/ml
Immunoprecipitation (IP) - recommended usage: 1-5 µg/ml
Flow Cytometry - recommended usage: 1-3 µg for 1 x 10⁶ cells
Immunocytochemistry/Immunofluorescence (ICC/IF) - recommended usage: 1-3 µg/ml

Target background

The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).

V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.

THE™ V5 Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with V5 tag peptide conjugated to KLH.

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Datasheet

Supplier

Genscript
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Specifications

Host species	Mouse
Immunogen	V5 tag peptide conjugated to KLH
Conjugate	Unconjugated
Form	Lyophilized
Storage buffer	Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification	Protein A affinity column
Isotype	Mouse IgG2a,κ
Clonality	Monoclonal
Clone ID	4C12E11