GST-tag Antibody [HRP], pAb, Rabbit - 40 µg

Art No | A00130-40

GST-tag Antibody [HRP], pAb, Rabbit - 40 µg

140.00 CHF

Brochure

Size: 40 µg

Description

This antibody can be used to detect GST-fusion proteins in almost expression systems including bacterial expression system, mammalian expression system and other systems.

Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA - recommended usage: 0.05-0.5 µg/ml
Western Blot - recommended usage: 0.1-1.0 µg/ml

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Western blot analysis of GST-tagged fusion protein using GST-tag Antibody [HRP], pAb, Rabbit (GenScript, A00130, 1 µg/ml). The signal was developed with DAB substrate (AMRESCO).

Predicted Size: 26 kD
Observed Size: 26 kD

Datasheet

Supplier

Genscript
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Specifications

Host species	Rabbit
Immunogen	Recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum
Species reactivity	Schistosoma japonicum
Conjugate	Horseradish peroxidase (HRP)
Form	Lyophilized
Storage Buffer	Lyophilized with PBS, pH 7.4, containing 1% BSAand 0.01% Thimerosal
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage	The antibody is stable in lyophilized form if stored at -20°C or below.The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification	Affinity chromatography
Isotype	Rabbit IgG
Clonality	Polyclonal
Clone ID	not applicable