UltraScript Reverse Transcriptase – Witec AG

UltraScript Reverse Transcriptase is a robust and thermostable modified MMLV reverse transcriptase engineered to enhance cDNA synthesis speed and yield with accurate transcript representation.

The enzyme allows highly sensitive reverse transcription of viral RNA sequences, making it an ideal component of RT-qPCR-based SARS-CoV-2 detection tests.

• Recommended product for reverse transcription step of COVID-19 diagnostic tests
• Sensitive and accurate reverse transcription of viral RNA sequences, including SARS-CoV-2
• Thermostable reverse transcriptase 45°C to 55°C
• Advanced RNase inhibitor
• High cDNA yields from as little as 4pg total RNA
• Accurate reverse transcription of GC-rich templates
• Sensitive detection of low copy number transcripts
• Advanced buffer chemistry including Mg and dNTPs

UltraScript Reverse Transcriptase is a thermostable and extremely active modified MMLV reverse transcriptase (RTase). The reaction temperature can be increased up to 55°C providing higher specificity and efficient transcription of GC-rich RNA regions with a high secondary structure. The RTase is blended with an advanced RNase inhibitor preventing degradation of RNA by contaminating RNase. UltraScript Reverse Transcriptase is not inhibited by ribosomal and transfer RNAs, making total RNA an ideal substrate.

The enzyme is supplied with a 5x buffer containing Mg, dNTPs, stabilizers and enhancers, optimised to generate high yield cDNA from as little as 4pg RNA. As oligos are not included, UltraScript Reverse Transcriptase provides the convenience and flexibility for users to define their own priming strategy depending on the type of analysis needed. UltraScript Reverse Transcriptase gives exceptional performance with gene-specific primers, oligo(dT) and random hexamers to produce high quality cDNA ideal for a variety of downstream applications.

Applications

• Random hexamer, oligo(dT) and gene-specific primers
• cDNA synthesis for PCR analysis, cloning, library preparation and Next Generation Sequencing
• Low copy number transcripts
• Viral RNA targets
• miRNA targets
• Efficient synthesis from total RNA or poly(A)+ RNA

Storage

The kit should be stored between -30°C and -15°C. Avoid prolonged exposure to light. If stored correctly the kit will retain full activity for 12 months. The kit can be stored at 4°C for 1 month. The kit can go through 30 freeze/thaw cycles with no loss of activity.