Art No | PB30.33
UltraScript 2.0 Reverse Transcriptase
from 229.50 CHF
15% OFF the entire range of PCR Biosystems PCR & qPCR Kits! Expires 31/12/2025
The Ultimate Reverse Transcriptase for Flawless cDNA
Description
UltraScript Reverse Transcriptase from PCR Biosystems is engineered for exceptional performance in cDNA synthesis. This enzyme is a modified MMLV reverse transcriptase that has been designed for increased thermal stability and robust cDNA synthesis, even with difficult templates. It offers high yields and full-length cDNA products from a wide range of RNA samples, including those with secondary structures or low abundance transcripts.
- Unbiased representation of 5’ and 3’ mRNA transcript ends
- Reduced RNase H activity
- Thermostable reverse transcriptase 45 °C to 55 °C
- Includes advanced RNase inhibitor
- High cDNA yields from as little as 4 pg template RNA
- Accurate reverse transcription of GC-rich templates
- Sensitive detection of low copy number transcripts
- Advanced buffer chemistry including Mg and dNTPs
- Both premixed or separate oligo cDNA synthesis kits available
UltraScript® Reverse Transcriptase is a thermostable and extremely active modified MMLV reverse transcriptase (RTase) for cDNA synthesis1. The reaction temperature can be increased up to 55 °C providing higher specificity and efficient transcription of GC-rich RNA regions with a high secondary structure. The RTase is blended with an advanced RNase inhibitor preventing degradation of RNA by contaminating RNase. UltraScript® Reverse Transcriptase is not inhibited by ribosomal and transfer RNAs, making total RNA an ideal substrate.
Applications
- Random hexamer, oligo(dT) and gene-specific primers
- cDNA synthesis for real-time PCR analysis
- cDNA synthesis for PCR analysis, cloning, library preparation and Next Generation Sequencing
- Low copy number transcripts
- Viral RNA targets
- miRNA targets
- Efficient synthesis from total RNA or poly(A)+ RNA
Select the right product for your application
| Primers | RNA Source | Intended Use | Recommended Product |
| Oligo(dT) & Random hexamers | Any | qPCR, cloning, NGS | PB30.11/PB30.15 |
| Random hexamers | Prokaryotic, Archaeal | qPCR, cloning, NGS | PB30.15, (PB30.12 user must supply primer) |
| Oligo(dT) | Eukaryotic | qPCR, cloning, NGS | PB30.15, (PB30.12 user must supply primer) |
| Gene specific primer | Any | Cloning, targeted qPCR, targeted NGS | PB30.12 (user must supply primer) |
Specifications
| UltraScript (200u/μL) with RNase inhibitor |
2 x 25μL: 10’000 Units 2 x 100μL: 40’000 Units |
| 5x UltraScript Buffer |
1 x 200μL: 10’000 Units 4 x 200μL: 40’000 Units |
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