Versatile electroporation system suitable for applications for gene, drug and protein delivery
Description
The ECM™ 830 is a square wave pulse generator designed for in vitro and in vivo electroporation applications. BTX square wave technology provides the advantage of efficient cell transfer and high cell viability for numerous applications. Applications of the versatile ECM 830 for gene, drug and protein delivery include mammalian cells, in vivo and ex vivo tissues, zebrafish tissue and embryos, nuclear transfer, embryo manipulation, plant protoplast and basic bacteria and yeast transformations.
The ECM 830 is a true laboratory workhorse with a 2-year warranty.
Monitoring Option
The Enhancer 3000 allows the researcher to monitor and track key electrical parameters used in electroporation applications. The electrical pulse data is captured as both a graphic display of the wave form and electrical output values following each experiment. This data can be stored on a memory stick or downloaded to a computer easily by using the USB port for potential analysis, documentation and validation purposes.
FEATURES
- Wide and accurate square wave voltages from 5 to 3,000 volts, with fine voltage discrimination
- Pulse durations from 10 μsec to 999 milliseconds
- Multiple pulsing capability and control of pulse intervals
- Preset protocols for commonly used mammalian cell lines and tissues
- User-defined protocols—unlimited ability to add and modify
- Safety—displays resistance measurements for each pulse with three layers of arc protection
- Data management—stores logs of every pulse delivered for QC and troubleshooting
- Ease of use—touch screen operation
- Can be used in combination with a wide array of BTX specialty electrodes and accessories to enhance your molecular and drug delivery for in vivo and ex vivo experiments
APPLICATIONS
- CRISPR transfections
- Mammalian cell transfection
- In vitro, in vivo, ex vivo and in ovo tissue transfection
- Nuclear transfer
- Ex plant tissue transfection
- Protoplast transfection
- siRNA libraries
Specifications
Display Type |
7inch color touchscreen |
Power Source Voltage |
100 to 240 VAC, 50 to 60 Hz, CAT II Power 500 W (Pulsed), 50 W (Idle) |
Fusing |
Type 3AG, 5 A, 250 V; Type T (time delay) |
Input |
110 to 240 VAC |
Charge Time |
LV <7 s, HV <4 s |
Pulse Length |
Range 10 μs – 999 μs LV Mode/ 1 μs resolution 1 msec – 999 msec LV Mode/ 1 msec resolution 1 sec – 10 sec LV Mode/ 0.1 sec resolution 10 μs – 600 μs HV Mode/ 1 μs resolution |
Voltage Range |
5 – 500 V LV Mode/ 1 V resolution 505 – 3,000 V HV Mode/ 5 V resolution |
Multiple Pulsing |
1 – 99 |
Pulse Interval |
100 msec – 10 sec |
Programmability |
Storage up to 1000 user defined protocols |
Safety |
Pre-pulse Sample Resistance check, Over-current Pulse Abort, Instrument Arc Protection |
Capacity |
3,775 μF LV, 85 μF HV |
Remote Operation |
Remote PC operation and footswitch operation available |
Accessories
The Electroporation Plate Handler creates a safe and reliable working area for processing electroporation plates. They are ideal for use with the ECM 630, ECM 830 and Gemini X2 systems.
The Electroporation Plate Handler creates a safe and reliable working area for processing electroporation plates. They are ideal for use with the ECM 630, ECM 830 and Gemini X2 systems.