DYKDDDDK-tag Antibody, pAb, Rabbit - 40 µg

Art No | A00170-40

DYKDDDDK-tag Antibody, pAb, Rabbit - 40 µg

140.00 CHF

Brochure

Size: 40 µg

Description

This Antibody recognizes DYKDDDDK tagged fusion proteins.

Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA - recommended usage: 0.05-0.5 µg/ml
Western Blot - recommended usage: 0.1-1.0 µg/ml
Western Blot - recommended usage: 1:100-1:1,000
Immunocytochemistry/Immunofluorescence (ICC/IF) - recommended usage: 5-20 µg/ml
Flow Cytometry - recommended usage: 1-3 µg for 1 x 10⁶ cells

Target background

The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.It has been used extensively as a general epitope tag in expression vectors.DYKDDDDK-tag Antibody, pAb, Rabbit can be used to detect DYKDDDDK-tagged proteins.

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Western blot analysis of DYKDDDDK-tagged fusion protein using DYKDDDDK-tag Antibody, pAb, Rabbit (GenScript, A00097, 1μg/ml).

The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (A00098; 1:20’000) and LumiSensor HRP Substrate Kit (L00221).

Predicted size: 49 kD
Observed size: 49 kD

Western blot analysis of DYKDDDDK tagged fusion proteins using DYKDDDDK-tag Antibody, pAb, Rabbit (GenScript, A00170, 1 μg/ml).

The signal was developed with One-Step Western Complete Kit (Rabbit) (Genscript L00204C).

Predicted size: 52 kD
Observed size: 52 kD

Western blot analysis of DYKDDDDK tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-DYKDDDDK-tag Polyclonal Antibody (GenScript, A00170, 1 μg/ml).

The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.

Immunofluorescent analysis of HEK293 cells transfected with Flag-tagged CD4 protein using DYKDDDDK-tag Antibody (GenScript, A00170, 10 μg/ml)

Datasheet

Supplier

Genscript
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Specifications

Host species	Rabbit
Immunogen	A synthetic peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH
Conjugate	Unconjugated
Form	Lyophilized
Storage Buffer	Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification	Affinity chromatography
Isotype	Rabbit IgG
Clonality	Polyclonal
Clone ID	not applicable