Art No | PB10.21
PCRBIO HS Taq DNA Polymerase - Novel hot-start technology (Incl. dNTPs)
from 140.00 CHF
PCRBIO Classic Taq is a highly purified recombinant Taq DNA Polymerase for all your everyday PCR applications including genotyping, screening and library construction.
Description
The enzyme and buffer system allow for superior PCR performance on complex templates such as mammalian genomic DNA.
- Increased PCR success rates with amplicons up to 6kb
- Advanced buffer chemistry – supplied with a 10x buffer containing 30mM MgCl2.
- High yields under standard and fast PCR conditions
- Efficient specific amplification from complex templates including GC and AT-rich sequences
PCRBIO Classic Taq is a robust enzyme for all your everyday PCR applications such as genotyping, screening and library construction. The enzyme and buffer system provide enhanced PCR speed, yield and specificity allowing for superior performance on complex templates such as mammalian genomic DNA. PCRBIO Classic Taq performs consistently well on a broad range of templates including both GC and AT-rich.
The enzyme is supplied with a 10x buffer containing 30mM MgCl2. PCR products generated are A-tailed and may be cloned into TA cloning vectors.
Applications
- Routine application PCR
- TA cloning
- Methylated DNA
- Standard and fast PCR
Storage
On arrival, products should be stored between -30 and -15°C. If stored correctly the kit will retain full activity for 12 months.
Specifications
| PCRBIO Classic Taq (5u/μL) |
1000 Units: 2 x 100μL 2000 Units: 4 x 100μL 6000 Units: 12 x 100μL |
| 10x PCRBIO Classic Buffer + 30mM MgCl2 |
1000 Units: 4 x 1mL 2000 Units: 8 x 1mL 6000 Units: 24 x 1mL |
| Reaction Volume |
50μL |
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