VSV-G-tag Antibody, pAb, Rabbit - 40 µg

Art No | A00199-40

VSV-G-tag Antibody, pAb, Rabbit - 40 µg

140.00 CHF

Brochure

Size: 40 µg

Description

This Antibody recognizes VSV-G tagged fusion proteins.

Applications

Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA - recommended usage: 0.05 -0.2 µg/ml
Western Blot - recommended usage: 0.1 -1.0 µg/ml
Western Blot - recommended usage: 1:100-1:1,000
Immunoprecipitation (IP) - recommended usage: 2-10 µg/mg of lysate

Target background

Rabbit Anti-VSV-G-tag Polyclonal Antibody is supplied as a 40 μg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide.It is purified by immunoaffinity chromatography.

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Western blot analysis of immunoprecipitates from VSV-G fusion protein lysates using Rabbit Anti-VSV-G-tag Polyclonal Antibody (GenScript, A00199).

Lane 1: Input control material for VSV-G fusion protein lysates
Lane 2: Negative control ‒ IP with isotype control antibody (GenScript, A01008)
Lane 3: Immunprecipitation with Rabbit Anti-VSV-G-tag Polyclonal Antibody (GenScript, A00199)

Western blot analysis of VSV-G tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-VSV-G-tag Polyclonal Antibody (GenScript, A00199, 1 µg/ml) The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.

Western blot analysis of VSV-G-tagged fusion protein using VSV-G-tag Antibody, pAb, Rabbit (GenScript, A00199, 0.1 µg/ml) The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensor HRP Substrate Kit (GenScript, L00221.

Predicted Size: 52 kD
Observed Size: 52 kD

Western blot analysis of VSV-G-tagged fusion protein using VSV-G-tag Antibody, pAb, Rabbit (GenScript, A00199, 1 µg/ml) The signal was developed with One-Step WesternTM Basic Kit (Rabbit) (GenScript, L00204).

Predicted Size: 52 kD
Observed Size: 52 kD

Datasheet

Supplier

Genscript
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Specifications

Host species	Rabbit
Immunogen	VSV-G epitope tag peptide YTDIEMNRLGK conjugated to KLH
Conjugate	Unconjugated
Form	Lyophilized
Storage buffer	Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 1 mg/ml
Storage	The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification	Affinity chromatography
Isotype	Rabbit IgG
Clonality	Polyclonal
Clone ID	Not applicable
Note	Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.