Size: 40 µg
Description
This Antibody recognizes c-Myc tagged fusion proteins.
Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
- ELISA - recommended usage: 0.05-0.5 µg/ml
- Western Blot - recommended usage: 0.1-1.0 µg/ml
Target background
Anti-c-Myc-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase.The antibody is suitable for detecting fusion proteins that contain c-Myc-tags and it is supplied as a 40 μg aliquot at a concentration of 1 mg/ml.
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
Specifications
| Host species |
Rabbit |
| Immunogen |
c-Myc epitope tag peptide EQKLISEEDL conjugated to KLH |
| Conjugate |
Horseradish peroxidase (HRP) |
| Form |
Lyophilized |
| Storage Buffer |
Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml |
| Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Affinity chromatography |
| Isotype |
Rabbit IgG |
| Clonality |
Polyclonal |
| Clone ID |
not applicable |