Webinars – Witec AG

SPEAKER

Nike Bahlmann, MS | Director of Global Support @ BioEcho Life Sciences

ABSTRACT

Nucleic acid extraction is a fundamental step in unlocking the genetic information contained within biological samples. Isolated nucleic acids are the basis for a multitude of analyses and applications spanning various scientific disciplines, such as RT-qPCR, dPCR, sequencing, and transcriptomics, among others. Thus, failure to isolate pure and high-quality nucleic acids frequently leads to experimental setbacks or misleading results.

Join for an insightful webinar in which the expert Nike Bahlmann will examine the intricate world of nucleic acid extraction. This comprehensive session will cover fundamental principles, and different extraction methods, including the most innovative technology on the market. Further, we will get insights into invaluable troubleshooting techniques. Learn about the importance of obtaining high-quality nucleic acids to get the best outcome from your experimental analysis.

At the end of this seminar, the participants will be able to:

• Identify the principles of the nucleic extraction process.
• List the different methods for the isolation of nucleic acids.
• Evaluate which method is the most appropriate to their sample type and downstream applications.
• Detect potential bottlenecks during the nucleic acid extraction procedure.

DNBSEQ-T7: Your gateway to Whole Genome Sequencing

Guest Speaker:

Dr. Erin Connolly-Strong | Chief Medical Officer @ Inocras

Abstract

• What digital SPR is and how it works.
• See how data quality and throughput are maximized using the latest advances in digital SPR to eliminate artifacts, optimize accuracy, and automate tedious, error-prone sample preps.
• See how researchers at Duke University utilize this technology to rapidly assess the binding kinetics of a covalent interaction between a biological ligand and its chemical analyte.

Make discoveries, not compromises. Discover how our SPR solutions can streamline your research.

Join this webinar with SPR experts from Nicoya and delve into the biomolecular applications of our next-generation SPR systems, including binding affinity and kinetics analysis, epitope characterization, library screening, and quantitation.

What you will learn:

• Overview of Nicoya's technology
• How Alto and OpenSPR streamline research workflows
• Applications of the Alto and OpenSPR platforms for characterizing biomolecular interactions, including:

• • Affinity and kinetics analysis
  • Epitope binning
  • Library screening
  • Quantitation

Summary

Metastases to the central nervous system from solid primary tumors are a frequent and fatal complication of cancer with limited therapeutic options. Despite the advent of immunotherapy, it remains largely unknown whether and how metastatic cells in the brain and surrounding cerebrospinal fluid (CSF)-filled leptomeninges interact with the immune system.

To approach these interactions in an unbiased manner, Jan Remsik and his colleagues profiled an unexpectedly heterogeneous population of cell types in human and mouse CSF with single-cell analytics. These samples were inherently challenging to process due to a relatively low number of cells from each human subject or mouse collection, extreme cell fragility, and contamination by myelin and other unwanted debris in the case of the mouse samples.

Since FACS-sorted samples suffered an unbearable amount of stress that prevented their processing, the team used the LeviCell 1.0 system to deplete the debris and enrich for viable cells in stress-free conditions.

Incorporating the LeviCell run into their pipeline resulted in the capture of sufficient numbers of high-quality cells for CITE-seq.

Such a proteogenomic approach, combining more than 100 cell surface signals with whole transcriptome sequencing, allowed Remsik and his colleagues to elucidate the response of the CSF immune system to cancer at an unprecedented resolution.

Innovative Nucleic Acid Extraction Technology with BioEcho Life Sciences

Nucleic acid extraction is a process used to purify DNA or RNA molecules from a biological sample. It is an essential step in many molecular biology applications, such as PCR, sequencing, Southern blotting, and cloning. Different techniques can be used to isolate nucleic acids, each with their own advantages and disadvantages

In this webinar, the expert from BioEcho will explain the principles behind each available method for nucleic acid extraction. Further, they will provide tips, such as sample processing, for optimizing the extraction procedure for downstream applications. Quality control and safety considerations will also be discussed.

In this webinar you will learn about:

• The history of nucleic acid isolation methods
• The principles of nucleic acid extraction
• EchoLUTION™: a single-step nucleic acid purification technology
• Nucleic acid quality as key to downstream applications

Asteria© single-cell RNA-seq kit

About this webinar

Most single-cell technologies today require cells to be manipulated one way or another, either by being frozen, fixed, or physically squeezed into microchannels or nanowells. Those requirements compromise the transcriptome with the cell altering gene expression in response and drifting further away from the physiological conditions. But novel solutions such as Asteria™ now enable fresh cells to be processed to preserve their transcriptome and thus the accuracy of the single-cell data.

Join Dr. Nicolas Fernandez, Director of Innovation, and Dr. Barbara André, Director of Bioinformatics, for the first half of this webinar to learn the impact of single-cell preparation on the transcriptome of your cells and how it can be prevented with the intuitive Asteria™ pipeline for fresh cell samples.

In the second half, Prof. Bertrand Blondeau will showcase his research using Asteria™ on fresh pancreatic islets tissues towards the understanding of Type 1 diabetes and the development of therapeutic treatments.

During this webinar will be discussed:

• Why processing fresh single-cell samples is better than fixed or frozen methods
• How to process fresh single-cell samples immediately in your lab
• Single-cell, single-tube, single-software: how to simplify your scRNA-seq experiments
• The application on pancreatic cell sample towards the understanding of Type 1 diabetes

Asteria™ Kit and Cytonaut™ Software:

Single-cell RNA-seq made accessible to any benchtop and any project

Planning single-cell experiments can be a tall order. Join Hélène Eckert, senior R&D Leader, and Barbara André, director of Bioinformatics, in this webinar to learn how the RevGel-seq technology can help you confidently design flexible, convenient, and efficient end-to-end single-cell RNA-seq studies.

During this seminar will be discussed:

• The technical concept behind the instrument-free technology RevGel-seq
• The flexibility of the Asteria™ scRNA-seq benchtop kit to ease single-cell logistics
• The performances of the Asteria kit on various cell types
• The accessibility of the companion data analysis software Cytonaut™ to all researchers
• And much more!

A Q&A session will follow the presentations, where you will have the chance to ask your single-cell related questions.

Register now to ensure your attendance!

Speakers

Hélène Eckert

Hélène Eckert has over 10 years of experience in molecular biology. After receiving her M.S. in Plant Genetics from the University of Nebraska-Lincoln (USA), she worked on plant genetic engineering at Iowa State University (USA) and Bayer Crop Science (Lyon, France). She then joined the Curie Institute in Paris, where she spent 6 years studying long noncoding RNAs in vertebrate development. She is a key leader in the development of the RevGel-seq technology now released as the Asteria™ scRNA-seq Kit.

Barbara André

Barbara André has 10+ years of experience in software R&D for biomedical applications. With an engineering background in computer science and applied mathematics, she received her PhD in medical image computing from Mines Paris Tech. She contributed to innovative prototypes and led the development of sustainable software products for the industry, especially in the domain of genetic analysis. At Scipio bioscience, she is leading the development of the Cytonaut™ single-cell data analysis software.

Inspiring the next generation of scientists

Access to the latest technology can make a world of difference in education today. Join us to hear how schoolteachers are integrating Thermo Scientific™ NanoDrop instruments into their classrooms and labs, providing students with the opportunity to learn on industry-leading technology. We will also cover the latest NanoDrop instrument innovations – from the 8-channel Thermo Scientific™ NanoDrop™ Eight to the entry-level Thermo Scientific™ NanoDrop™ Lite Plus Spectrophotometer.

Gain unique insights into the landscape of science education by:

• Listening to teachers describe how they use NanoDrop instruments to teach molecular biology
• Learning how NanoDrop advancements improve downstream experiment success by identifying potentially harmful contaminants in DNA/RNA and protein samples
• Discovering how the new NanoDrop Lite Plus instrument makes critical sample information more accessible

Who should attend:

• Teachers of future scientists (high schools, technical schools, community colleges)
• University professors
• Academic researchers

Detecting emerging viral variants through rapid antibody discovery and characterization

Overcoming the barriers to antibody discovery with on-cell mAb screening
and surface plasmon resonance

What you'll learn:

• Challenges of emering viral variants
• New strategies for viral detection
• Accelarting antibody discovery with rapid screening & characterization
• Emerging technologies for mAB engineering

Join Nicoya in a conversation with Scott Dessain (OCMS) and Marko Jovic (Nicoya), who will discuss the application of novel mAb screening technology and surface plasmon resonance (SPR) for rapid discovery and characterization of variant-specific antibodies, and will delve into the future of immunodiagnostics.

Utilizing RNA and DNA plate-seq workflows for low-input or rare single-cell genomics

Single-cell studies in recent years have revealed that organisms are composed of thousands of unique and unrepeatable cell types. Conducting single-cell genomic analyses using next-generation sequencing (NGS) methods, similar to low-input sequencing, has traditionally been challenging due to the limited amount of genomic DNA present in single cells. This limitation becomes even more pronounced when the studied population is small, such as in the case of rare cells. High-fidelity (HiFi) and high-quality genomic DNA amplification is essential for single-cell sequencing, which is strongly dependent on the quality of the isolated cells.

Therefore, the method used to isolate single cells becomes an important consideration for ensuring cell viability and nucleic acid integrity. Namocell’s patented gentle sorting and cell enrichment technology overcomes the aforementioned challenges to provide a higher quantity and quality of cell samples for sequencing. As a result, a complete and accurate picture of the studied samples can be achieved.

Whether you’re a beginner or expert in single-cell analysis, join this informative webinar to learn about the considerations, challenges and new technologies for low-input or rare single-cell genomics, followed by a discussion on the latest tips for low-input NGS.

Detecting emerging viral variants through rapid antibody discovery and characterization

Overcoming the barriers to antibody discovery with on-cell mAb screening
and surface plasmon resonance

What you'll learn:

• Challenges of emering viral variants
• New strategies for viral detection
• Accelarting antibody discovery with rapid screening & characterization
• Emerging technologies for mAB engineering

In this webinar, we will introduce the Fusion fluorescence and chemiluminescence systems manufactured by Vilber.  

The Fusion systems are the ideal modular, multimodal imaging platforms designed for molecular biology laboratories. The application spectrum covers Chemiluminescence, multicolour fluorescence from UV to infrared and all applications in gel documentation.

The advanced technology used in the Fusion Systems results in the most sensitive instruments in the market,  combined with an unmatched ease-of-use. It comes with a license free software for image editing and quantification.

VILBER is a leading life science company developing and manufacturing fluorescence and chemiluminescence imaging systems for applications ranging from small animal to cell biology research. Founded in 1954, Vilber is a leader in the molecular imaging sector, equipping more than 20,000 laboratories worldwide. An estimated 60,000 researchers use their products every day in over 100 countries.

Presenters

• Alexis Frances | Application Specialist @ Vilber
• Dr. Tim Hander | Area Sales Manager @ Witec

Single cell sorting and isolation has traditionally been a bottleneck in the workflows for single cell cloning, single cell genomics, rare cell isolation, synthetic biology and other applications. Existing techniques such as fluorescence-activated cell sorting (FACS) often leads to compromised cell viability and poor outgrowth, contamination, clogging, in addition to the operational complexity and high maintenance requirement, while manual limiting dilution is inefficient, unreliable and highly labor-intensive. As such, there has been an urgent need for fast and easy single cell dispensing technology that is at the same time gentle to the cells and can preserve their viability and integrity. There is also a need for quick sorting of tens of thousands of cells for single cell genomics, such as sample prep for 10x Genomics workflow.

In this presentation, the speaker will discuss an innovative technology to address these needs. It combines the benefits of three key technologies: microfluidics, flow cytometry and liquid dispensing. The unique combination of these techniques enables users to accomplish single cell sorting and dispensing in one step, providing fast, easy and gentle sorting and isolation of single cells. This will simplify and empower a number of single cell applications, including cell line development, single cell genomics and proteomics, cell line engineering and CRISPR, monoclonal antibody development, synthetic biology, and rare cell isolation such as circulating tumor cells (CTCs) and circulating fetal cells.

Register for this webinar to discover how this technology can help optimize a single cell workflow and deliver desired results.

Presenter: John Lee, Applications Scientist | Namocell

Watch this webinar to learn more about a state-of-the-art, one-of-a-kind, disruptive 4D bioprinting technology from Poietis:

• Short Introduction Witec AG by Dr. Tim Hander
• Introduction of the Bioprinting System by Antoine Jacquot - Poietis
• Application focus - soft tissue models, 4D bioprinting, organoids, chips/devices by Dr. Mikael Garcia - Poietis
• Workflow – instrument operating in details by Antoine Jacquot - Poietis

The NGB-R platform integrates automation and robotics, numerous online sensors, and machine-learning based analysis software. In addition, it integrates all bioprinting techniques (laser-assisted bioprinting, bioextrusion, and micro-valve bioprinting), a world’s first in the bioprinting market.

Focusing on a cytocentric approach, tissues grow in a more natural environment, leading to proper morphogenesis and predictable biological processes, including cell differentiation, migration, multiplication, and metabolic processes.

Presenters

• Antoine Jacquot | Sales Manager Poietis
• Dr. Mikael Garcia | Business Application Manager Poietis
• Dr. Tim Hander | Product Specialist Genomics & Single Cell Technologies Witec

• Looking to take your lab automation to the next level?
• Looking to improve success rates, reduce development time, and go-to-market faster?
• Looking to increase throughput capabilities and reduce costs?

Learn how, Alto, the world’s first digital benchtop SPR instrument powered by microfluidics and AI can help you address all the above. Alto uses 2uL sample volume, has zero maintenance, and produces industry-leading data quality.

This year, the NanoDrop One spectrophotometer has received two major updates – features that allow 21 CFR Part 11 compliance and the ability to distinguish DNA from RNA.

Join us while we explore how electronic signatures, audit trails, and user account access have been incorporated into the NanoDrop One platform using the Thermo Scientific Security Suite software. You’ll also learn how the Thermo Scientific Acclaro Sample Intelligence Technology algorithms have been updated to identify RNA contamination in DNA and DNA contamination in RNA.

Key learning objectives

• Compliance with 21 CFR Part 11 doesn't have to be difficult
• How to integrate your NanoDrop One into software that allows for 21 CFR Part 11 compliance
• How to distinguish between DNA and RNA using UV/Vis spectroscopy

Who should attend?

• Molecular biologists working with nucleic acids and proteins in their research
• Researchers who perform qPCR tests
• Biopharmaceutical research and quality control labs

Certificate of attendance

All webinar participants can request a certificate of attendance, and a learning outcomes summary document for continuing education purposes.

About the Event

The COVID-19 pandemic disrupted research worldwide, causing many scientists to put their projects on hold. As labs and facilities begin to slowly open up again, many of us are asking where to go from here. Whether it be planning your next big breakthrough or that side project you've had in the back of your mind, it's important to have the right tools to get the data you need. And with so many innovations in biomolecular technology, it's common to find yourself unsure of which techniques are best suited for your research.

To help you navigate the many biomolecular interaction techniques available as a researcher, Nicoya has put together this webinar to dive into which technique best suits the direction of your projects. Nicoya will be discussing the importance of binding interaction data and providing an overview of the strengths and weaknesses of multiple techniques. If you have ever done co-immunoprecipitation or western blots, or considered working with ITC, MST, BLI, or SPR, this is a perfect opportunity to understand which technique is the best fit for your projects.

You'll Learn How to

• Why reviewers are looking for binding data in publications
• What technique best suits your research
• An overview of Co-IPs, MST, ITC, BLI and SPR
• Newest advancements in biomolecular characterization methods

Nicoya will be showcasing the strengths of common binding techniques and helping you understand which technique you need for your next publication. It is the perfect opportunity to learn about new technologies and how to make the most out of your resources that are available to you!

In this webinar we will discuss and compare several single cell isolation techniques including manual limiting dilution, flow cytometry and Namocell's unique single cell dispenser.

The results of single cell cloning using all three techniques with several different cell lines will be shared.

We will demonstrate the advantages of using an automated gentle single cell isolation instrument can improve overall cloning workflows.

Presenter:

• Michael Brem - Senior Scientist - Cell Line Development @Cytovance Biologics Inc.
• Abe Couse - VP Marketing and Business Development @Namocell inc.

Join Namocell for an informative talk providing an overview of Namocell's single cell dispensers

and explanation of their benefits over conventional approaches to single cell isolation.

They will also demonstrate their use in several single cell application workflows.

On September 18th, 2018 at 4pm (EDT)

you have the opportunity to join the online event

"Protein Sample Evaluation using the NanoDrop™ One UV Vis Spectrophotometer"

Presenter:

Voula Kodoyianni, PhD, MS
Product Marketing Manager | UV-Vis and Fluorescence Instruments | Thermo Fisher Scientific

We know from VOC that 83% of our customers quantify proteins with a spectrophotometer at least once per week. There is a lot of helpful information in this webinar for scientists who work with proteins. Concepts covered and clarified are:

• Protein mass extinction coefficients vs molar extinction coefficients
• Blank vs background correction
• How to use “sample type options”
• “Good” and “bad” buffers for protein A280
• How to customize their instrument using the Protein Editor and Dye Editor
• How the Proteins and Labels application quantifies both the carrier protein and the FLR label
• and more...

If you are interested, please register online.