ALTO 16-channel benchtop SPR system

Art No | ALTO16

ALTO 16-channel benchtop SPR system

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Accelerate Drug Discovery with Digital SPR

Description

Simplifying interaction analysis with Digital SPR

Alto is the world’s first Digital Surface Plasmon Resonance (SPR) instrument to integrate digital microfluidics (DMF) with nanotechnology-based biosensors. DMF dramatically increases the ease of use of SPR while providing high throughput, high-quality binding affinity and interaction data.

The 16-channel instrument design:

Eliminates fluidics maintenance with a disposable cartridge that is compatible with a wide range of sample types, including crude media
Saves 200x the sample volume, obtaining full binding kinetics curves from only 2µL
Reduces hands-on time by 70% by automating all sample and buffer movements, including serial dilutions

Explore the versatility of Alto

Assay modules available

Sample compatibility

Molecule types

Applications

Basic research (mechanism of signaling, action analyses)
Biopharmaceutical drug discovery (protein, antibody, peptide, oligo drug candidates)
- Hybridoma, phage display screening
- Antibody-antigen, Antibody-Fc receptor kinetics
- Developability testing (charge variant impact on potency)
- Epitope binning
Biopharmaceutical drug development
- Antibody Titer
- Potency assessment (charge variants, biosimilars, stability testing)
Vaccine R&D
- Antigen characterization
- Antibody immune response in serum
- Virus particle characterization
Applied research
- Reagent characterization (quality assessment)

Miniaturization with integrated cartridge

Alto’s innovative integrated cartridge combines 16 parallel sensors with all of the samples, including reagents and buffers, into a cartridge to simplify assay set up. The single-use cartridge supports up to 48 samples. Serial dilutions of each sample are performed onboard, allowing for measuring up to 240 total interactions in one cartridge ― all without user intervention. Once the assay is complete, cleaning up is as simple as removing the cartridge!

Digital microfluidics: new approach to SPR assays

Digital microfluidics (DMF) is a liquid handling technology for manipulating nanoliter-sized droplets across an array of electrodes. Contained within the Alto Cartridge, DMF brings the samples and buffers to the SPR sensor without needing any of the pumps, tubing, or flow cells used by traditional label-free systems, ultimately eliminating the need for maintenance. Alto is compatible with crude samples such as serum, lysate, and cell culture supernatant.

Beyond dramatically increasing the ease of use, the DMF cartridge design additionally breaks traditional SPR limitations by:

Removing human error in sample dilutions to increase quantitative accuracy
Decoupling flow rate and sensor position from dispersion to enhance data quality
Disconnecting interaction time sample volume to increase assay flexibility

Software designed for any skill level

Alto’s comprehensive software, the Nicosystem, provides a centralized hub for acquisition and analysis of real-time binding data, while offering you the flexibility of accessing and sharing your experiments from anywhere.

Explore Nicosystem options for your research needs and budget today.

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APPLICATION NOTES

Kinetic and epitope characterization of influenza antiviral targets using Alto™ digital surface plasmon resonance

Label-free Characterization of ImmTAC® Bispecific Molecules for T-Cell Redirecting Immunotherapy using Alto™

Label-Free Characterization of the Interleukin-6 Receptor and
Anti-IL-6R Antibody Drug Tocilizumab using Alto™ Digital SPR

Comparison of Single-Sequence and
Multi-Sequence Kinetic Analysis of a
Protein-Antibody Interaction on Alto

Protein interaction analysis using Alto™ digital surface plasmon resonance

Characterization of Biomolecular Interactions using Alto:
Comparison to Conventional SPR

Mass Transport Limited (MTL) Kinetics Analysis with Alto™ Digital SPR

Supplier

Nicoya Lifesciences Inc.
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Specifications

Performance	Association Rate (kon): Up to 1×10⁹ 1/M*s Dissociation Rate (koff): 1×10⁻⁵–1.0 1/s Affinity Range (KD): pM – mM
Core Detection Technology	Localized SPR fiber optic biosensors
Data Channels	16
Analytes	Up to 48 analytes/cartridge
Sample Volume	2μL
Reference:Standard	1:1
Dilutions	Automated serial dilutions
Unattended Run Time	24+ hours
Instrument Size \| Weight	43 x 35 x 52cm \| 23.1kg