Accelerate Drug Discovery with Digital SPR
Description
Simplifying interaction analysis with Digital SPR
Alto is the world’s first Digital Surface Plasmon Resonance (SPR) instrument to integrate digital microfluidics (DMF) with nanotechnology-based biosensors. DMF dramatically increases the ease of use of SPR while providing high throughput, high-quality binding affinity and interaction data.
The 16-channel instrument design:
- Eliminates fluidics maintenance with a disposable cartridge that is compatible with a wide range of sample types, including crude media
- Saves 200x the sample volume, obtaining full binding kinetics curves from only 2µL
- Reduces hands-on time by 70% by automating all sample and buffer movements, including serial dilutions
Explore the versatility of Alto
Applications
Miniaturization with integrated cartridge
Alto’s innovative integrated cartridge combines 16 parallel sensors with all of the samples, including reagents and buffers, into a cartridge to simplify assay set up. The single-use cartridge supports up to 48 samples. Serial dilutions of each sample are performed onboard, allowing for measuring up to 240 total interactions in one cartridge ― all without user intervention. Once the assay is complete, cleaning up is as simple as removing the cartridge!

Digital microfluidics: new approach to SPR assays
Digital microfluidics (DMF) is a liquid handling technology for manipulating nanoliter-sized droplets across an array of electrodes. Contained within the Alto Cartridge, DMF brings the samples and buffers to the SPR sensor without needing any of the pumps, tubing, or flow cells used by traditional label-free systems, ultimately eliminating the need for maintenance. Alto is compatible with crude samples such as serum, lysate, and cell culture supernatant.
Beyond dramatically increasing the ease of use, the DMF cartridge design additionally breaks traditional SPR limitations by:
- Removing human error in sample dilutions to increase quantitative accuracy
- Decoupling flow rate and sensor position from dispersion to enhance data quality
- Disconnecting interaction time sample volume to increase assay flexibility

Software designed for any skill level
APPLICATION NOTES
Kinetic and epitope characterization of influenza antiviral targets using Alto™ digital surface plasmon resonance
Label-free Characterization of ImmTAC® Bispecific Molecules for T-Cell Redirecting Immunotherapy using Alto™
Label-Free Characterization of the Interleukin-6 Receptor and
Anti-IL-6R Antibody Drug Tocilizumab using Alto™ Digital SPR
Comparison of Single-Sequence and
Multi-Sequence Kinetic Analysis of a
Protein-Antibody Interaction on Alto
Protein interaction analysis using Alto™ digital surface plasmon resonance
Characterization of Biomolecular Interactions using Alto:
Comparison to Conventional SPR
Mass Transport Limited (MTL) Kinetics Analysis with Alto™ Digital SPR
Specifications
Performance |
|
Core Detection Technology |
Localized SPR fiber optic biosensors |
Data Channels |
16 |
Analytes |
Up to 48 analytes/cartridge |
Sample Volume |
2μL |
Reference:Standard |
1:1 |
Dilutions |
Automated serial dilutions |
Unattended Run Time |
24+ hours |
Instrument Size | Weight |
43 x 35 x 52cm | 23.1kg |