Clara™ HRM Mix – Witec AG

See Your Data Clearly. Melt With Confidence.

Description

Clara™ HRM Mix is anext generation qPCR mix developed to give maximum specificity to your high resolution melt (HRM) curve analyses. Accurately detect genetic mutations, quickly identify genotypes based on SNPs, or reliably calculate percent methylation of a target region with HRM analysis.

Accurate distinction of SNP classes I-IV
Reliably quantify methylation of target sequences
Super-senstitive product melt curves for distinct allele profiles
Compatible with all HRM-suitable real-time instruments
Powered by PCRBIO Taq DNA Polymerase

Applications

Accurate SNP genotyping
Gene scanning
CpG methylation analysis

Superior HRM Mix

Clara™ HRM Mix is our latest high performance qPCR mix for high resolution melt curve (HRM) analysis. The mix contains our PCRBIO Taq DNA Polymerase, dNTPs, MgCl2 and our third-generation, DNA interacalating dye, SyGreen 2. This non-inhibitory DNA binding dye allows the highly senstitive melt curve profile generation for superior HRM analysis.

SNP detection

Proprietary smart screen technology has enabled the development of an enzyme buffer with novel composition that guarantees excellent SNP differentiation capabilities. Use Clara™ HRM Mix to distinguish all classes of SNP:

Class I: C to T and G to A
Class II: C to A and G to T
Class III: C to G
Class IV: A to .

Methylation studies

Clara™ HRM Mix can also be used to quantify CpG methylation in a target sequence by combining HRM qPCR with bisulfite sequencing. HRM analysis on the bisulfite treated versus a corresponding, untreated, control sample allows the estimation of the target’s cytosine methylation percentage, because methylated sequences will have a higher CG content compared to unmethylated targets following bisulfite treatment.

Material Safety Data Sheet (PDF)

Product manual (PDF)

Instrument Compatibility Tool

PCRBIO Tm Calculator