LeviCell 1.0 System - Cell Isolation and Enrichment

Art No | 1000001

LeviCell 1.0 System - Cell Isolation and Enrichment

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Description

Unlock previously inaccessible samples.

Increase your sample viability up to 99%
Reduce dead cells and debris by up to 5x
Start with cell counts as low as 8K

The advanced LeviCell® 1.0 system provides groundbreaking cellular analysis technology not seen in over thirty years. Now, for the first time ever scientists, researchers, and labs can examine, analyze, and deliver true biological signatures without altering, stressing or damaging cells - while preserving your original samples. LeviCell delivers true biology for real cellular insights to power oncology, neuroscience, immunology, and pharma drug research.

Any Size, Any Type

Compatible with any sample source, cell type, and cell media
Scalable to large cells, clusters, and organoids
Unaffected by cellular and extracellular debris

Untouched and Label-Free

No dyes or labels of any kind required
Low pressure, minimal cell stress
Limited cell activation and expression profile distortion

Simplified Workflow

Simple 3-steps, 20-minute workflow
No additional sample prep, purification, or debris removal required
Run-to-run reproducibility without calibration

Sample Types

Dissociated Tissue
Blood
Biopsies
Saliva
Urine
Fine Needle
Aspirates
Swabs
Cell Culture
Fixed Tissue
Lavages

Cell / Particle Types

Immune cells
Cancer cells
Lung cells
PBMCs
Stem cells
Neurons
Embryos
Organoids
Small Animals (C. elegans)
Cell clusters
Fetal cells

Applications

NGS
Single-cell sequencing
FACS
Proteomics
Transfection
Gene editing
Microarray analysis
Cell culture

The LeviCell Cartridge

The LeviCell cartridge is a single-use consumable designed to perform sample enrichment on the LeviCell system. The cartridge is easily inserted and removed from the system, providing an easy sample loading and collection process. With a sample path that is 100% contained within the cartridge, sample cross-contamination and system cleaning requirements are a thing of the past.

Optically clear, inert, biocompatible plastic
Sterile, single-use design
Accommodates samples up to 350µm in diameter

LeviCell Workflow Advantages

Methods

LevitasBio has developed an innovative levitation technology platform that uses less than 1 psi of pressure to enable a touch-free, label-free, three-step enrichment process that takes only 20 minutes to complete.

Live cell populations levitate much higher than dead cells and debris, allowing for clear separation and collection by the LeviCell, as shown. The fluorescent dyes used in the provided image were used solely to highlight the separation process and are not required to use the LeviCell system.

LeviCell’s gentle separation maintains maximum cell viability, enabling the preservation of cell population representation integrity for downstream assays such as single-cell analysis. The simple three-step process streamlines the workflow and minimizes the risk of contamination and damage from multiple instances of handling and washing, consequently maximizing enrichment for each cell type.

Step 1

Sample Introduction

Sample pipetted into the LeviCell cartridge.

Step 2

Automated Label-Free Sorting

Precision density gradients drive levitation based on cells physical properties.

Step 3

Sample Collection

Selected populations collected into separate ports for further analysis or use.

Results

Primary samples were processed on the LeviCell system and using the most common bead-based approach from a leading supplier. The results presented here for both methods were achieved by manually counting the live and dead cells to calculate the yields and viabilities.

Revolutionary viability and yields

LeviCell demonstrated the successful removal of dead cells and debris from a variety of primary samples while maintaining the original representation of the populations found within the sample. Furthermore, the LeviCell was able to enrich a low starting number of live cells to a final viability of >90%, exhibiting a capacity for scalability regardless of starting number of cells. 100,000 cells from each of two dissociated tumor cells (DTCs) were successfully processed and enriched to a final viability of >90% through removal of dead cells and debris in 3 steps and 20 minutes.

Viability vs. Cancer & Fraction

$Viability-vs-Cancer-and-fraction$

Superior performance over bead-based methods

The ovarian sample was split into two fractions of 100,000 cells and each fraction independently processed using either the LeviCell system or a popular bead-based dead cell removal kit. The output from each enrichment method was analyzed by manual cell counting using a Trypan Blue stain and a hemocytometer. LeviCell clearly outperforms the standard bead method in both Yield and Viability, while providing a faster, simpler and much more accommodating workflow when working with challenging samples. Additionally, LeviCell maintains the original cellular representation of the native sample without affecting the gene expression profile or activation state in even the most sensitive cell types.

Viability vs. Sort Method & Sort

Yield vs. Sort Method

Maximum levitation height difference between differentiated and undifferentiated adipocytes was achieved using 30 mM of Levitation Agent (Figure A). As the concentration of Levitation Agent increased, overall levitation height of both populations increased while the distance between differentiated and undifferentiated cells decreased.

No negative effects on post-processing population representation

The data on the right show how LeviCell’s gentle cell separation approach enables the study of notoriously delicate and sensitive primary cell types without compromising the integrity of the original cell populations. Sorted and unsorted ovarian DTC samples were blocked with TruStain human Fc Block for 15 minutes at RT then stained with 5 μL each of anti-CD45, anti-CD19, anti-CD11b, and 10 μL of anti-CD3, for 30 minutes at room temperature. Samples were washed with FACS buffer (0.5% BSA in PBS) once before analyzing on a Sony SH800S cytometer. The LeviCell output shows a similar amount of CD3+, CD19+, and CD11b+ cells within the CD45+ lymphocyte population while the sample processed by the bead method shows reduced proportions of CD3+ and CD11b+ cells.

No effect on the post-processing activation state

Macrophages, one of the most sensitive cell types, demonstrates the gentleness of the LeviCell system. The data below confirms there are no adverse side effects on cells’ post-processing activation state.

RAW264.7 cells were treated with IFN-g and IL-4 in order to polarize the macrophages into either the M1 or M2 stage. The samples were then processed on the LeviCell system. qRT-PCR was than performed to examine the expression of M1 specific gene (iNOS) or M2 specific (Arg1) in these cells to show that their activation states were not altered by the LeviCell. No differences in gene expression were observed between the input and output cells for the activation states.

LeviCell’s unique levitation platform enables scientists to significantly reduce costs (financial and otherwise) by reducing the amount of time to enrich for specific cell types from hours and days to mere minutes.

The three-step process simplifies the workflow and minimizes the need for highly skilled technicians and complicated single-cell preparation protocols. Because the LeviCell system does not use any high pressure, labels, dyes, or antibodies to isolate cells, alteration of expression profile. LeviCell’s closed environment also eliminates the risk of contamination and cell death due to manual manipulation. Separating live cells from dead and debris is as easy as finding the line of delineation between them.