LeviCell 1.0 System - Label-Free Cell Enrichment – Witec AG

Any Size, Any Type

• Compatible with any sample source, cell type, and cell media
• Scalable to large cells, clusters, and organoids
• Unaffected by cellular and extracellular debris

Untouched and Label-Free

• No dyes or labels of any kind required
• Low pressure, minimal cell stress
• Limited cell activation and expression profile distortion

Simplified Workflow

• Simple 3-steps, 20-minute workflow
• No additional sample prep, purification, or debris removal required
• Run-to-run reproducibility without calibration

LevitasBio has developed an innovative levitation technology platform that uses less than 1 psi of pressure to enable a touch-free, label-free, three-step enrichment process that takes only 20 minutes to complete.

Live cell populations levitate much higher than dead cells and debris, allowing for clear separation and collection by the LeviCell, as shown. The fluorescent dyes used in the provided image were used solely to highlight the separation process and are not required to use the LeviCell system.

LeviCell’s gentle separation maintains maximum cell viability, enabling the preservation of cell population representation integrity for downstream assays such as single-cell analysis. The simple three-step process streamlines the workflow and minimizes the risk of contamination and damage from multiple instances of handling and washing, consequently maximizing enrichment for each cell type.

Step 1

Sample Introduction

Sample pipetted into the LeviCell cartridge.

Step 2

Automated Label-Free Sorting

Precision density gradients drive levitation based on cells physical properties.

Step 3

Sample Collection

Selected populations collected into separate ports for further analysis or use.

Results

Primary samples were processed on the LeviCell system and using the most common bead-based approach from a leading supplier. The results presented here for both methods were achieved by manually counting the live and dead cells to calculate the yields and viabilities.

Revolutionary viability and yields

LeviCell demonstrated the successful removal of dead cells and debris from a variety of primary samples while maintaining the original representation of the populations found within the sample. Furthermore, the LeviCell was able to enrich a low starting number of live cells to a final viability of >90%, exhibiting a capacity for scalability regardless of starting number of cells. 100,000 cells from each of two dissociated tumor cells (DTCs) were successfully processed and enriched to a final viability of >90% through removal of dead cells and debris in 3 steps and 20 minutes.

Viability vs. Sort Method & Sort

Yield vs. Sort Method

Maximum levitation height difference between differentiated and undifferentiated adipocytes was achieved using 30 mM of Levitation Agent (Figure A). As the concentration of Levitation Agent increased, overall levitation height of both populations increased while the distance between differentiated and undifferentiated cells decreased.

RAW264.7 cells were treated with IFN-g and IL-4 in order to polarize the macrophages into either the M1 or M2 stage. The samples were then processed on the LeviCell system. qRT-PCR was than performed to examine the expression of M1 specific gene (iNOS) or M2 specific (Arg1) in these cells to show that their activation states were not altered by the LeviCell. No differences in gene expression were observed between the input and output cells for the activation states.