ALTO 16-channel benchtop SPR system – Witec AG

Art No | ALTO-16
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The world’s first digital high‑throughput benchtop SPR system.

Description

Surface Plasmon Resonance Powered by Digital Microfluidics.

Alto is the world’s first SPR instrument to integrate digital microfluidics (DMF), artificial intelligence (AI), and nanotechnology to provide high quality, label-free interaction analysis. DMF technology enables all fluidics to be disposable, allowing for precise handling of 2uL sample volumes, crude sample compatibility and zero maintenance.

Alto’s compatibility with automation for both hardware and software provides
sample-in/answer-out data, industry leading throughput, and 24/7 runtime. Every aspect of Alto is designed with user-friendliness and accessibility in mind - from the flexible 16-channel design to the intuitive data analysis platform.

Alto is capable of supporting operation in GXP and 21 CFR Part 11 in compliance with regulatory demands.

 

  • Industry-Leading Data Quality
    Minimize noise and dispersion; maximize sensitivity
    The fastest rise times at <0.1s
  • High-Throughput Powered by Automation and AI
    16 channels, intelligent assay design/optimization, automated serial dilutions
    Fully compatible with plate & liquid-handling robots
  • Sample-Friendly
    Only 2μL required for full kinetics
    Analyze crude samples with ease
  • User-Friendly
    Free of high-maintenance fluidic components and obsolete software

 

Alto is the world’s first SPR instrument to integrate digital microfluidics (DMF), artificial intelligence (AI), and nanotechnology.

DMF technology enables all fluidics to be disposable, allowing for precise handling of 2μL sample volumes, crude sample compatibility and zero maintenance. Alto’s compatibility with automation for both hardware and software provides sample-in/answer-out data, industry leading throughput, and 24/7 runtime.

Every aspect of Alto is designed with user-friendliness and accessibility in mind - from the flexible 16 channel design to the intuitive data analysis platform.

 

 

Applications:

  • Kinetics/Affinity Characterization
  • Kintetics/Affinity Screening
  • Competition Assays
  • Yes / No Binding
  • Concentration Analysis
  • Thermodynamics
  • Epitope Mapping/Binning

Compatible with:

  • Proteins
  • Antibodies
  • Nucleic Acids
  • Small Molecules
  • Carbohydrates
  • Lipids
  • Cells
  • Viruses
  • Nanoparticles

What is Digital Microfluidics?

DMF is a liquid-handling technology capable of accurately controlling and manipulating discrete nanodroplets with electricity on a standard microwell plate.

 

Why DMF?

DMF fluidic handling technology removes the need for any physical pumps, valves, or tubes and replaces them with a low cost disposable cartridge, providing higher data quality and less hands-on time.

 

How does SPR work with DMF?

We’ve integrated our proprietary localized surface plasmon resonance (LSPR) sensors into DMF cartridges – creating 16 independently addressable channels on a single cartridge.

 

Advantages of using DMF-powered SPR

  • Decoupling of flow rate and sensor position from dispersion, increasing data quality

 

  • 500X less sample volume required— get full kinetics from a 2μL sample volume

 

  • Decoupling of interaction time from sample volume, increasing assay flexibility

 

  • Automated serial dilutions—reducing human error and saving time

 

ALTO Cartridges

ALTO Cartridges

Alto Cartridges have completely revolutionized the interaction of fluidics and sensors for SPR.

A Cartridge is a well-plate sized consumable that integrates reagent/sample storage, fluid handling, and sensor surfaces. Samples, buffers and reagents are contained in wells on the cartridge, and dispensed towards the sensor in software-controlled nanodroplets.

Precise volumes and automated serially diluted concentrations interface with a fiber optic sensor tip, manufactured with the same quality and consistency (<2% CV) as our existing localized SPR sensors.

 

ALTO Surface Chemistries

Surface Chemistries

  • Carboxyl for any amine group using EDC/NHS coupling
  • NTA for histidine tagged targets
  • Streptavidin for biotin tagged targets
  • Biotin for streptavidin coupled targets
  • GST for GST tagged targets
  • Protein A for IgG based antibodies
  • Amine for any carboxyl group using EDC/NHS coupling
  • Liposome Binding (LIP) for liposomes/membrane proteins
  • Hydrophobic (MEM) for lipid monolayer
  • Thiol for thiol or maleimide tagged targets
  • Gold for non-functionalized and for custom surface chemistry development and targets with thiol groups
Alto Software Suite

Alto Software Suite

Alto features an end-to-end software suite that is powered by AI, built directly into the instrument, and leverages the power of the cloud. Flexible and intelligent assay design and optimization enables efficient planning of experiments. Serial dilutions are fully automated and controlled by software, reducing hands-on time and human error. With cloud connectivity, real-time data and wizards will clearly communicate experiment progress and can be accessed by any licensed device connected to the internet.

Features

  • Intuitive and easy-to-operate built in PC with customizability based on level of SPR experience
  • Guided setup, quality control and shutdown procedures
  • Software Wizards to guide experiment set-up, optimization and quality control
  • Full test automation with the ability to create, save and load test templates
  • Real-time 16-channel data of binding interactions featuring error warnings & experiment suggestions
  • Seamless and flexible transition of real-time data into data analysis software
  • Multiple instruments can be networked together and controlled easily from 1 interface

Supplier

Nicoya Lifesciences Inc.
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Specifications

Sample Volume

2μL

Rise Time

<0.1s

Association rate (kon)

<1e9 1/(M*s)

Dissociation Rate (koff)

1e-6 to 1.0 (1/s)

Affinity Range (KD)

1e-12 to 1e-4 (M)

Automation

Compatible with robots for 24/7 runtime

Compliance

GXP and 21 CFR Part 11*

*Alto is capable of supporting operation in GXP and 21 CFR Part 11 in compliance with regulatory demands.

Operating Environment

15°C–35°C
20–80% RH Non-Condensing 0m–2000m Altitude

# Optical Channels

16 independently addressable

Reference : Standard

1:1 standard, any combination possible

Ligands

Up to 16

Analytes

Up to 64 Analytes/Cartridge

Dilutions

Automated Serial Dilutions

Crude Compatibility

Yes

Hands on Time

<30 mins

Temperature Control

8°C–50°C in sensor area

8°C in sample storage area

Instrument Size

35W x 50.5D x 40.4H (cm)

Instrument Weight

18kg

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