Art No | A00186-100
THE™ His Tag Antibody, mAb, Mouse - 100 µg
520.00 CHF
Size: 100 µg
Description
THE™ V5 Tag Antibody [HRP], mAb, Mouse recognizes C-terminal tagged fusion proteins, and it slightly recognizes N-terminal V5 tagged fusion proteins.
Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
- ELISA - recommended usage: 0.05-0.1 µg/ml
- Western Blot - recommended usage: 0.2-1 µg/ml
Target background
V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
GenScript THE™ V5 Tag Antibody [HRP], mAb, Mouse is THE™ V5 Tag Antibody, mAb, Mouse (A01724) conjugated with Horseradish peroxidase (HRP). The antibody is suitable for detecting the expression level of V5 Tagged fusion proteins.
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
Specifications
| Host species |
Mouse |
| Immunogen |
V5 tag peptide conjugated to KLH |
| Conjugate |
Horseradish peroxidase (HRP) |
| Form |
Lyophilized |
| Storage buffer |
Lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.01% thimerosal |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL |
| Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Mouse IgG2a,κ |
| Clonality |
Monoclonal |
| Clone ID |
4C12E11 |
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