Size: 100 μg
Description
THE™ NWSHPQFEK Tag Antibody [HRP], mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins.
Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
- Western Blot - recommended usage: 0.2-1 µg/ml
- ELISA - recommended usage: 0.001-0.002 µg/ml
Target background
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin.
NWSHPQFEK (Strep II tag) is a nine amino acid peptide with high specificity and affinity towards streptavidin.
GenScript THE™ NWSHPQFEK Tag Antibody [HRP], mAb, Mouse is THE™ NWSHPQFEK Tag Antibody (A01732) conjugated with Horseradish peroxidase (HRP).
The conjugated antibody is suitable for detecting the expression level of NWSHPQFEK Tagged fusion proteins.
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
Specifications
Host species |
Mouse |
Immunogen |
NWSHPQFEK peptide conjugated to KLH |
Conjugate |
Horseradish peroxidase (HRP) |
Form |
Lyophilized |
Storage buffer |
Lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.01% thimerosal |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Purification |
Protein A affinity column |
Isotype |
Mouse IgG1,κ |
Clonality |
Monoclonal |
Clone ID |
5A9F9 |