THE™ HA Tag Antibody [FITC], mAb, Mouse - 100μg

Art No | A01621

THE™ HA Tag Antibody [FITC], mAb, Mouse - 100μg

420.00 CHF

Brochure

Size: 100 µg

Description

THE™ HA Tag Antibody [FITC], mAb, Mouse recognizes C-terminal, N-terminal, and internal HA tagged fusion proteins.

Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Immunocytochemistry/Immunofluorescence (ICC/IF) - recommended usage: 0.5-2 µg/ml
Flow Cytometry - recommended usage: 0.5 µg for 1 x 10⁶ cells

Target background

GenScript THE™ HA Tag Antibody [FITC], mAb, Mouse is FITC conjugated THE™ HA Tag Antibody, mAb, Mouse (A01244) purified from mice ascites by Protein A affinity column.

This product is suitable for detecting the expression level of HA fusion proteins by various immunoassays.

Absorption Wavelength: 492 nm
Emission Wavelength: 518 nm
Fluorochrome/Protein Ratio: >3 moles FITC per mole antibody

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

A01621-THE-HA-Tag-Antibody-FITC-mAb-Mouse

Flow cytometric analysis of transfected HEK293 cell expressing HA-tagged protein using THE^TMAnti-HA-tag [FITC] Monoclonal Antibody (GenScript, A01621; shaded histogram) or with a negative control antibody (open histogram).

Datasheet

Supplier

Genscript
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Specifications

Host species	Mouse
Immunogen	A synthetic peptide YPYDVPDYA coupled to KLH
Conjugate	Fluorescein (FITC)
Form	Lyophilized
Storage buffer	Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification	Protein A affinity column
Isotype	Mouse IgG1,κ
Clonality	Monoclonal
Clone ID	5E11D8