SUMO-tag Antibody (4G11E9), mAb, Mouse - 40μg – Witec AG

SUMO-tag Antibody (4G11E9), mAb, Mouse specifically reacts with fusion proteins containing SUMO epitope tags.

Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

• ELISA - recommended usage: 0.05-0.2 µg/ml
• Western Blot - recommended usage: 0.5-1 µg/ml

Target background

SUMO tags are emerging as a viable alternative for increasing both the expression and solubility of otherwise hard-to-express proteins. The SUMO tag can be cleanly excised using SUMO protease, which recognizes the conformation of SUMO protein rather than a specific sequence within SUMO. The SUMO system can be used effectively in both prokaryotic and eukaryotic systems.

GenScript SUMO-tag Antibody (4G11E9), mAb, Mouse is suitable for detecting fusion proteins that contain a SUMO-tag. The SUMO-tag Antibody (4G11E9), mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with recombinant yeast SUMO protein.

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.