Avi Tag Antibody, mAb, Mouse - 40 μg – Witec AG

Avi Tag Antibody, mAb, Mouse recognizes C-terminal Avi tagged fusion proteins and slightly recognizes N-terminal Avi tagged fusion proteins in Western Blot.

Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

• Western Blot - recommended usage: 0.2-1 µg/ml
• ELISA - recommended usage: 0.02-0.05 µg/ml
• Flow Cytometry - recommended usage: 1-3 µg for 1 x 10⁶ cells
• Immunocytochemistry/Immunofluorescence (ICC/IF) - recommended usage: 1-3 µg/ml

Target background

Avi tag is a biotin-acceptor peptide, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain.

Avi Tag Antibody is a useful tool in analysis of Avi fusion proteins.

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.