LeviSelect Mouse Tissue RBC Depletion Kit (10 rxn) – Witec AG

Unwanted tissue red blood cells (RBC) removal now can be done lysis-free

The LeviSelect Mouse Tissue RBC Depletion Kit has been designed to deplete up to 5 million mature erythrocyte and erythroid precursor cells per reaction after a mechanical or enzymatic tissue dissociation while simultaneously enriching for live cells.

The depleted tissue red blood cells (RBCs) remain bound to the cartridge during a run on the LeviCell® systems. At the same time, the remaining viable, untouched cells will be separated from dead cells in the suspension and then collected in the top fraction output of the cartridge. The bottom fraction will consist primarily of dead cells, as the depleted erythroid cells will be immobilized inside the cartridge.

RBCs are identified and bound using a biotinylated mouse RBC antibody cocktail. Streptavidin magnetic nanospheres then bind these biotin-labeled RBCs. When loaded into the LeviCell systems cartridge placed within the magnetic field in the LeviCell platforms, the nanosphere-coated cells are depleted from the suspension, leaving the cells of interest in suspension.

Kit components:

• 1 tube of LeviSelect m RBC SAV Nano- spheres (100 uL)
• 1 tube LeviSelect m RBC Ab Cocktail (10 uL)
• 2 tubes of 1X LeviSelect Buffer (1.8 mL), sufficient for 10 reactions