LeviSelect Human CD4 T Cell Enrichment Kit

Art No | 1004007

LeviSelect Human CD4 T Cell Enrichment Kit

260.00 CHF

Brochure

Size: 10 rxns

Targeted and viable cell enrichment in a single step

Description

Enrich label-free human CD4 T cells from a peripheral blood mononuclear cells (PBMC) sample concurrently with viable cell enrichment on a LeviCell system. Viable human CD4 T cells can be used for various downstream applications. Non-CD4 T cells are magnetically labeled and remain immobilized inside the LeviCell cartridge.

Includes Biotinylated-Antibody Cocktail, StrepAvidin Nanospheres, Buffer, and Protocol
No centrifugation or washing steps required
Average 20 min run for targeted viable cell enrichment and removal of dead, dying cell or debris

Kit includes:

1 tube of LeviSelect hCD4 SAVNanospheres (100 uL)
1 tube LeviSelecthCD4 Ab Cocktail (100 uL)
2 tubes of 1XLeviSelect Buffer (1.8 mL), sufficient for 10 reactions

LeviSelect Immune Kits support target selection of the immune subpopulations required for downstream cell analysis or further subsetting. You can enrich hematopoietic populations quickly without lengthy workflows or potential cell activation.

CD3 Enrichment
CD4 Enrichment
CD8 Enrichment
Pan B Enrichment
Pan Monocyte Enrichment
NK Cell Enrichment

How it works

LeviSelect products combine Levitation Technology with targeted selection of specific cell types. Targeted and viable cell enrichment are performed simultaneously on the LeviCell system. By combining the natural density and magnetic susceptibility of cells within a paramagnetic compound with the addition of antibodies conjugated to nanospheres of different densities, cells can levitate at different heights. This process allows the separation of specific cells of interest from unwanted cells during a single LeviCell run.

Identifying and enriching cells of interest from mixed cell populations is fundamental to studying disease models and basic cell biology. Levitation Technology provides the gentlest cell enrichment method, preserving target-specific cells of interest unbiasedly.

Target Selection Workflow

Enabling target selection on the LeviCell system is just as easy as viable cell enrichment. Prepare your sample by adding Levitation Agent and LeviSelect reagents. Enrich for cells of interest with the LeviCell system. Collect viable cells for downstream applications.

LeviSelect Depletion and Enrichment Kits

LeviSelect Kits provide a fast and efficient method to deplete a specific cell type away from a mixture of cells or remove several cell-specific populations to enrich for a target cell of interest (negative selection). In both kit types, the target cell of interest remains label-free, ensuring cells are not perturbed.

Obtain gentle enrichment of viable target-specific populations, which remain untouched within the LeviCell cartridge. Now, you can achieve dying or dead cell removal – plus removal of debris – in a single LeviCell run.