Neutral Protease NB GMP Grade - = 100 U (DMC)

Art No | N0002936

Neutral Protease NB GMP Grade - = 100 U (DMC)

1'420.00 CHF

Brochure

Size: ≥ 100 DMC

Alone or in Combination with other GMP Collagenases

Description

Neutral Protease is a metalloprotease that can cleave peptide bonds in proteins such as collagen, proteoglycan, fibronectin, elastin etc. Due to these properties, it is suitable for the isolation of a broad range of cell types by tissue dissociation.

In combination with Collagenase NB 1 GMP Grade, Neutral Protease NB GMP Grade is especially suited for isolation of islets of Langerhans from human pancreas. However, Neutral Protease NB GMP Grade can also be used in combination with Collagenase NB 6 GMP Grade for applications like tissue engineering and transplantation into humans which have to meet GMP requirements .

Supplier

Nordmark Pharma GmbH
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Specifications

Origin	Clostridium histolyticum
Composition	Contains chromatographically purified neutral protease
Molecular weight	34 kDa
Special features	Manufactured according to intl. GMP guidelines
Microbiological examination	Endotoxin^: Sterility (Ph. Eur.) Absence of Clostridia TAMC (Total Aerobic Microbial Count) ≤ 10/Vial TYMC (Total Yeasts/ Moulds Count) ≤ 10/Vial Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
Activities	Neutral Protease ≥ 100 U/Vial (DMC assay^) Neutral Protease ≥ 0.50 U/mg lyophilisate (DMC assay^) ** Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS
Storage conditions	+2 °C to +8 °C
Shipping conditions	Cooled on blue or dry ice depending on the destination
Intended use	For clinical applications