Size: ≥ 2000 DMC
For Pancreatic Cell Isolation and other GMP Applications
Description
Collagenase NB 1 GMP Grade is a highly purified collagenase grade.
This product is used for isolation of sensitive cells and guarantees high yields and viability.
In combination with Neutral Protease NB GMP Grade, Collagenase NB 1 GMP Grade is especially applicable to isolation of pancreatic islets of Langerhans. Collagenase NB 1 GMP Grade is recommended for applications like tissue engineering and transplantation into humans which have to meet GMP requirements.
Specifications
Origin |
Clostridium histolyticum |
Composition |
Collagenase class l and class ll1 1 Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091 |
Molecular weight |
70 – 120 kDa (collagenases) |
Special features |
Manufactured according to intl. GMP guidelines |
Microbiological examination |
Endotoxin*: * Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)). |
Activities |
Collagenase ≥ 2000 U/Vial (PZ** activity according to Wünsch, 25 °C) ** **PZ Unit (acc. to Wünsch2): 2 Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler’s Z. Physiol. Chem. 333, 149-51 |
Storage conditions |
+2 °C to +8 °C |
Shipping conditions |
Cooled on blue or dry ice depending on the destination |
Intended use |
For clinical applications |