For Cell Isolation of Rodent Pancreas and Tumors
Description
Collagenase NB 8 Broad Range is a chromatographically purified enzyme resulting in a high collagenolytic activity. This product has an increased collagenase activity compared to Collagenase NB 4 Standard Grade, Collagenase NB 4G Proved Grade and Collagenase NB 7D Gentle Grade. The additional enzymatic activities like clostripain, trypsin-like activity and neutral protease, as well as the endotoxin level are reduced.
Collagenase NB 8 is suitable for a broad range of different applications in tissue dissociation such as isolation of rodent pancreatic islets of Langerhans, human cardiomyocytes, tumor cells, muscle cells, osteoblasts and porcine hepatocytes.
Related Products
If you are dealing with poor cell yields, try collagenases with higher side activities like Collagenase NB 4 Standard Grade and Collagenase NB 4G Proved Grade.
Specifications
Origin |
Clostridium histolyticum |
Composition |
|
Molecular weight |
70 – 120 kDa (collagenases) |
Microbiological examination |
Endotoxin: ≤ 100 EU/mg |
Activities |
Collagenase activity: ≥ 0.90 U/mg (PZ activity acc. to Wünsch, 25 °C) |
Storage conditions |
+2 °C to +8 °C |
Shipping conditions |
At ambient temperature |
Intended use |
For research use only |