Size: 1g
For Human ADSCs, Chondrocytes and Stem Cell Passaging
Description
Collagenase NB 5 Sterile Grade is a natural enzyme mixture that contains collagenolytic and additional enzymatic activities including clostripain and neutral protease and is sterile according to European Pharmacopoeia. The balanced ratio of these activities ensures gentle and efficient tissue dissociation.
Collagenase NB 5 is applicable to tissue dissociation such as
- isolation of human and rodent hepatocytes
- chondrocytes
- neuronal and endothelial cells
- fibroblasts
- cells from adipose tissue (e.g. ADSCs)
- stem cells and their passaging
- rodent osteoblasts
Specifications
Origin |
Clostridium histolyticum |
Composition |
Contains class I and class II collagenase and a balanced ratio of proteolytic side activities |
Molecular weight |
70 – 120 kDa (collagenases) |
Activities |
Collagenase activity: ≥ 0.10 U/mg (PZ activity* acc. to Wünsch, 25 °C) |
Storage conditions |
+2°C to +8°C |
Intended use |
For research use only |