Size: 5mL (50% slurry)
GenScript’s Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.
Description
Protein A Resin FF can also be used for immunoprecipitation of proteins, protein complexes or antigens.
The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to have a high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin FF is greater than 40 mg human IgG/ ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc.
Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly at their Fc region. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding to recombinant protein A, the Fab region is available for binding antigens.
Specifications
Ligand |
Recombinant Streptococcal protein A expressed in E. coli |
Number of IgG binding sites per ligand |
5 |
M.W. of ligand |
approx. 34 kDa |
PI of ligand |
5.17 |
Degree of substitution |
approx. 5mg protein A/mL settled resin |
Static binding capacity |
>40mg human IgG/mL settlet resin |
Matrix component |
Agarose, 4% highly cross-linked |
Average particle size |
90 μm (45-165 μm) |
Storage solution |
20% Ethanol |
Storage conditions |
2-8°C |
Shelf life |
12 months when stored unopened |