Anti-DYKDDDDK G1 Affinity Resin

Art No | L00432

Anti-DYKDDDDK G1 Affinity Resin

Size:

from 390.00 CHF

DYKDDDDK tag is an octapeptide tag that can be added to a protein using recombinant DNA technology.

Description

It can be fused to the N-terminus or C-terminus of a target protein to facilitate detection and purification.

GenScript Anti-DYKDDDDK G1 Affinity Resin is designed for the purification of DYKDDDDK-tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells. The DYKDDDDK-tagged protein in the cell lysate can specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin. Nonspecific bindings are eliminated by stringent washing steps, and the target protein is finally eluted with high recovery rate.

High DYKDDDDK binding capacity and ultralow levels of non-specific binding
Multiple elution methods for diverse protein characteristics and different requirements
Used for purification and immunoprecipitation of DYKDDDDK-tagged protein or protein complex

Comparison between Anti-DYKDDDDK IP Resin and Anti-DYKDDDDK G1 Affinity Resin.

		Anti-DYKDDDDK G1 Affinity Resin (L00432)	Anti-DYKDDDDK IP Resin (L00425)
Immunoprecipitation (IP)		√	√
Purification		√	n/a
Elution	Alkaline elution	√	n/a
	Alkalescent elution	√	n/a
	Acid elution	√	n/a
	Neutral elution	√	n/a
	Competitive elution	√	n/a
	SDS-PAGE loading synthesize buffer	√	√
Regeneration		4-10 times	not recommend
Binding volume		large scale	mini-volume (<1ml)

Selection Guide Resins (PDF)

Supplier

Genscript
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Specifications

Product content	50% settled resin in TBS with 0.02% sodium azide
Matrix	4% cross-linked agarose
Average bead size	90 μm
Ligand	Mouse monoclonal antibody against DYKDDDDK tag, clone G1
Binding capacity	Approximately 1 mg DYKDDDDK-tagged protein per ml settled resin
Storage and stability	Store at 2-8 °C for up to 12 months. Do not freeze the resin.
Resin reuse	The resin can be recycled for at least 4 times. If maintained properly, the resin can be reused 10 times with minimum loss of binding capability
Elution method	Acid, alkaline, neutral, peptide competitive or PAGE loading buffer NOTE: Elution with SDS-PAGE loading buffer causes denaturation of the G1 antibody.
Reagents compatibility	Compatible with commonly used reagents at certain concentrations