NEWTON 7.0 BIO - Plant Imaging – Witec AG

ABSOLUTE NIST TRACEABLE CALIBRATION

Absolute NIST Traceable Calibration Imaging is based on traceable references provided by the National Institute of Standards and Technology (NIST). It allows precise quantification of specific biomolecules (such as proteins) in the sample of interest and provides benefits such as accuracy, reproducibility, and standardization.

IT ALL MAKES SENSE

Absolute quantification allows researchers to compare and integrate data from different studies or laboratories. Unlike relative quantification, which compares the amount of an analyte between two or more samples, absolute quantification provides a precise value for the amount of substance in the sample, expressed in absolute units.

DETECTION CAPABILITIES

• Luciferase expression
• Green fluorescent protein (GFP)
• red-shifted GFP expression
• Chlorophyll phosphorescence
• NIR illumination

CHOICE OF SAMPLES

• Small plants
• Fruits
• Vegetables
• Leaves
• Cyanobacteria
• Green algae
• Seedlings
• Insects
• Blossoms
• Roots
• Mosses
• Lichens
• Fungi
• Cell & tissue cultures

INCREDIBLE EASE OF USE

• No user limits
• Intuitive user interface
• One click to get the image
• Auto-exposure & automatic illumination control
• Adjustable stage

QUALITY ONLY

• Very robust design for long-term durability
• Made of aluminium and stainless steel (no plastic)
• Elegant, modern and compact design to fit your work environment

UNRIVALLED SENSITIVITY AND SHARPNESS

The NEWTON 7.0 BIO proprietary optics have been specifically developed for macro imaging with high light collection capacity, incorporating a unique combination of high numerical aperture and long working distance. Our system is equipped with a high-resolution scientific CCD camera which means better performance in pattern recognition. Bright fluorescence observation can be performed in a rapid scanning mode that shortens exposure times and minimizes specimen damage.

MULTISPECTRAL AND FLUORESCENCE IMAGING

Our dual magnetron filter technology ensures transmission above 90% and very narrow band cutting. The time to get the image is drastically reduced and sensitivity is increased. Our system accommodates 8 excitation channels in the visible RGB and NIR spectrum (from 400 to 900nm). Signals can be overlaid so that several reporters can be visualized simultaneously. The very tight LED spectrum is additionally constrained with a very narrow excitation filter. This means less background in the images (ultra-low noise imaging) of your sample and a higher signal to noise ratio to detect the weakest signals.

BEST CAMERA PERFORMANCE

The NEWTON 7.0 BIO is equipped with a high-sensitivity scientific CCD camera with a proprietary lens offering the widest aperture in the industry (f/0.70) as well as a deep camera cooling down to -90°C to ensure the best signal to noise ratio (which is ideal for faint luminescence applications). With up to 4.6 image resolution, obtain the sharpest details from your samples.

HEIGHT ADJUSTABLE PLANT STAGE

Enjoy a 20×20 FOV to visualize large plants! The rotating stage is motorized on the Z axis to get closer to the CCD camera. It can be inclined by 15° on the X/Y axis and easily controlled from the software, avoiding time consuming manipulation.

BIOPHOTON EMISSION

Monitor cellular activity in living organisms, such as plant stress, non-invasively and in real time by detecting and visualizing the light they emit.

LUCIFERASE & GFP EXPRESSION

Investigate the behavior and interactions of specific proteins in real-time and in their native environment thanks to GFP expression. Track gene expression, understand plant physiology, characterize and distinguish between various microbial infections or cloned plant screening using luciferase.

DELAYED FLUORESCENCE

Measure the time delay between light absorption and re-emission to study the photosynthetic activity and health of plants. Reveal the presence of hidden stresses that are not detectable by traditional methods, which is very useful for crop management and plant breeding.

CHLOROPHYLL FLUORESCENCE

Capture the fluorescence emitted by chlorophyll molecules within leaves to assess the photosynthetic efficiency and stress level within plants. Get critical insights into the impacts of environmental stresses on plant health and productivity and address global food security challenges.