Art No | INS-INC-HUM-CO2-D
Stage-top incubator digitally controlled CO2 regulation
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A Microscopy System that Grows with Your Research.
Description
Andor's Benchtop Confocal delivers amazing images at the push of a button. This plug and play laser confocal system has been designed with cost, performance and accessibility in mind, ensuring hassle-free, high-quality 2D & 3D imaging for busy researchers.
- Benchtop multimodal Imaging System
- Instand confocal: Blur-free imaging
- Widefield imaging
- Differential phase contrast & brightfield
- Super resolution ready
- In-field upgradable imaging modes
«We found the Andor benchtop confocal incredibly easy to set up and use. It produces great images and has become a real workhorse in our lab, handling different specimens from live zebrafish larvae to tissue sections and organoids.”
Prof. Dr. med. Ruxandra Bachmann-Gagescu | Molecular Life Science/University of Zurich
Fluorescence immunohistochemistry analysis of the retinal structure in a 10-day-old zebrafish larva. Nuclei labeled with DAPI in blue, opsins are represented in green and photoreceptor outer segments are delineated in magenta.
Picture credited by Department of Molecular Life Science | University of Zurich
Fluorescence immunohistochemistry examination of the mature zebrafish retina. Nuclei are depicted with DAPI staining (blue), acetylated tubulin is visualized in green and photoreceptor outer segments are highlighted in magenta.
Picture credited by Department of Molecular Life Science | University of Zurich
Stitched image of an intact larval brain, displaying nuclear DNA labeling in blue, acetylated tubulin immunostaining in green and primary ciliary are highlighted in magenta.
Picture credited by Department of Molecular Life Science | University of Zurich
Fluorescence immunohistochemistry of iPSC-derived cortical neurons at 70 days of differentiation.
Nuclei are shown with DAPI staining (blue), MAP2 is stained green, TUBB3 is stained magenta and B4-Spectrin is stained white.
Picture credited by Department of Molecular Life Science | University of Zurich
Fast and Easy Confocal Microscopy in any Lab
BC43 is a super-compact unit that is rich in features and benefits, making it the ideal microscope for early-stage researchers and experienced microscopists alike. With no requirement for a darkroom, researchers can access laser confocal microscopy technology on the same bench as their samples. This, coupled with the fast learning curve, allows individual users and entire labs to become much more productive, without compromising results.
Andor is the first microscope manufacturer to offer an IQ / OQ quality control programme which ensures that all BC43 microscopes operate within tightly controlled parameters, providing confidence that your data and experiments are reproducible in the future.
Confocal, Widefield & Transmitted Light Microscopy All-In-One
The BC43 benchtop confocal utilizes imaging technology from our award-winning Dragonfly confocal system and provides fast and remarkably flexible imaging. BC43 allows routine imaging from brightfield to confocal without the hassle.
Powerful and Intuitive Acquisition Software
At the heart of BC43 is a software that is not only feature-rich and flexible to your needs, it is easy-to-use as well:
- Imaris rendering engine – stunning real-time 3D images
- Clear and concise user-interface – acquire images in no time
- Multi-dimensional imaging protocols – advanced 3D time lapse or multi-position montage made easy
Comprehensive Image Analysis Software with AI Tools
All Andor Benchtop Microscopes come with the introductory Imaris Quantify package for users to:
- Visualise their 2D/3D/4D images in the world’s leading interactive microscopy image analysis software.
- Identify and characterize objects within their images via traditional intensity-based and AI methods.
- Report counts, morphology metrics, intensity values, and many more statistics to find differences across images and experiments.
- Create high-resolution snapshots and multi-dimensional movies with ease.
Additional Imaris modules are also available including tools for tracking and reporting motility metrics, quantifying cells and their intracellular organelles, and tracing neurons for reporting their specific characteristics.
Feedback from BC-43 workshop
“I have to admit, I was impressed with the BC43 system. It’s incredibly user-friendly and the speed at which it acquires images for the 3D projection is great! Not to mention, the image quality was also good. If the BC43 system becomes available at our facility, I would absolutely love to use it. I can see how it would make our work so much easier and more efficient.”
Drosophila Embryos
Pictures credited by Biozentrum, University of Basel
Drosophila
Pictures credited by Biozentrum, University of Basel
Confocal Microscopy
Widefield Fluorescence Microscopy
Transmitted Light Microscopy
Confocal Microscopy
Capture superb confocal images at least 10 times faster than point scanner-based systems with the Andor Benchtop Confocal!
BC43 is a prime solution for 3D imaging of thick samples. A high sensitivity, high-speed imaging camera makes it perfect for live-cell confocal imaging.
A motorised XY stage allows multiple point acquisition, large organism montages and multipoint scanning resulting in outstanding productivity from a single imaging experiment.
Widefield imaging:
- Offers the gentlest illumination mode for samples that are super-sensitive to light
- Detects lower sample signals than in confocal mode
- Offers the fastest imaging speeds to capture dynamic processes in live cells
Use ClearView™ GPU to remove sample blur and enhance resolution, ensuring the best quality images.
Transmitted Light Microscopy
BC43 also delivers two different transmitted light options for amazing imaging flexibility:
- Use Brightfield Mode to visualise the development of larger organisms whose structures naturally deliver high contrast images
- For thinner samples and/or those with higher transparency, use Differential Phase Contrast (DPC) mode to draw out their structure
You can also mix and match modes like DPC and Widefield for even more imaging flexibility!
High-Speed Confocal Imaging for Biologists
The BC43 Benchtop Confocal has been developed in collaboration with leading biologists; for use across life science applications, and is designed to be part of a single room workflow. BC43 will bring instant clarity and depth to your research, whatever your sample, in ways previously only found in larger, more complex and expensive imaging systems.
Cancer
BC43 addresses the broad scales and types of imaging protocols and analysis related to cancer research. From subcellular and cellular live cell experiments to large tissue samples and organoids requiring deeper imaging.
- Image monolayer and 3D cell cultures.
- Follow cell dynamics (intercellular behaviour, migration and division)
- High productivity imaging of tissue preparations
- Analyse movement, cell lineage, count cells, features and more.
- Boost your unit with the Super-resolution module for detailed spatial understanding of actin filaments, microtubule networks, distribution of vesicles and lysosomes etc
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Cell Biology
BC43 conquers the diverse range of protocols that Cell Biology experiments demand: from a simple 2D snapshot of a cell to understanding dynamic processes with time-lapse.
BC43’s unrivalled clarity and speed will save you time without compromising resolution (X,Y or Z) or field of view.
- Confocal mode for fast, high-resolution imaging of tissues
- Widefield mode for higher sensitivity and maximum speeds
- Built in GPU-based ClearView™ technology boosts image clarity
- Boost your unit with the Super-resolution module for detailed spatial understanding of actin filaments, microtubule networks, distribution of vesicles and lysosomes etc,
Core Facilities
Andor’s Benchtop Confocal is a compact, but fully featured system for routine imaging. It can be the imaging workhorse you are looking for, freeing up your sophisticated systems with advanced tools for those who need them.
Simplicity of use has been designed into BC43 to reduce training time and ongoing support of users, enabling labs and facilities to become more efficient.
Researchers will rapidly become competent in acquiring excellent 3D images, and higher multi-dimensional experiments on their own.
- Four excitation lines (405, 488, 561 & 640nm)
- Up to 5 imaging objectives (exchangeable)
- Multiple imaging modes offers flexibility whatever the sample and whatever your budget and needs
Developmental Biology
Cut through the challenges of your 3D world and capture images in perfect clarity – from the very first rounds of cell division to the fully developed organism.
Extract crisp 2D images or explore stunning 3D volumes in a fraction of the time you’re used to. Andor’s Benchtop Confocal delivers fast, high-resolution imaging of developing model organisms such as zebrafish and Drosophila.
- Image deeper than conventional fluorescence microscopes
- Boost productivity 10-fold compared with traditional confocals
- No sacrifices to resolution, field of view or 3D detail for speed
Neuroscience
Break through the burden of countless images demanded by your neuroscience research with a ten-fold speed boost, compared to conventional confocal microscopes. Flexible imaging modes cover sensitive live imaging of neuronal processes to large scale imaging of entire neuronal networks in 3D.
- Outstanding real-time 3D visualisation for immediate insight
- Discover more imaging live with high temporal resolution
- Dedicated image analysis tools with Imaris: automated process tracing, auto depth, spine detection and more
- Add super-resolution for fine detailed imaging of neuronal processes.
Tissue Imaging
Capture stunning 2D and 3D images from live tissue models to whole cleared-tissue preparations.
BC43 is a true workhorse instrument for large sample imaging, capturing in a tenth of the time of conventional confocal systems and imaging deeper than widefield epifluorescence with deconvolution or digital clearing.
- Time-saving, large-view, high-resolution imaging of tissue.
- Capture huge samples with tiled volume imaging and stitching
- Innovative analysis tools available with Imaris: machine learning object classification, spatial analysis, volume and morphology and many more.
Specifications
| Microscope Unit - Imaging Modes |
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| Microscope Unit - Imaging Methods |
Single colour, multicolour, z-stacking (volume), time-lapse, multi-position, multi-well, montage and 2/3D stitching |
| Microscope Unit - ClearView GPU |
Clears image of non-specific sample background signal and improves resolution beyond the normal optical limits |
| Camera - Resolution |
6.5 um pixel; 2048 x 2000 pixels (4.1MP) |
| Camera - QE2 |
Up to 82% |
| Camera - Field of view (mm) |
18.4mm (diagonal) |
| Camera - Cooling |
0°C |
| Camera - Images |
16-bit, monochrome |
| Illumination - Fluorescence |
4 fixed wavelengths of
|
| Illumination - Transmitted light |
Broad spectrum visible light LED |
| Optics - Objective Lens Nosepiece |
Motorised 5 position turret |
| Optics - Objective Magnifications |
BC43 is supplied with 2x objective for sample overview. Select additional supported objective lenses from 10x to 60x magnification |
| Optics - Precision motorised x,y stage |
Travel Range = 100mm x 80mm Resolution = 10nm |
| Optics - Z-Control & Focus |
Range = 14.5mm |
| Optics - Autofocus "Seek & Lock" Technology |
Sample “Seek & Lock”. Finds focal plane for new sample and maintains focus stability during time-lapse experiments. |
| Optics - Sample Vessels Supported |
Optimal imaging through glass, for imaging through plastic vessels, consult your sales representative |
| Optics - Incubation (option) |
Stage-top incubator. Sliding lid for easy sample access and exchange. Objective heater for oil-immersion objectives. |
| Workstation |
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