Art No | INS-INC-HUM-CO2-D
Stage-top incubator digitally controlled CO2 regulation
ask for a quote
Widefield Fluorescence, Confocal and Super Resolution All in One!
Description
Andor's expanded range of benchtop microscopes prioritizes cost, performance, and accessibility.
Meet the new BM42 and BC43 WF widefield microscopes – they're fast, easy-to-use, compact, and affordable. The BC43 WF also offers an in-field upgrade path so the system can evolve with your research.
Our original, award-winning benchtop solution, the BC43 CF, is a high-end confocal microscope that captures outstanding images with a single click.
Rounding out the portfolio is the BC43 SR, a microscope that delivers super-resolution data faster and more easily than ever.
All models are backed by our IQ/OQ quality control program for guaranteed performance and include Imaris image analysis to help you derive meaningful insights from your images.
A Benchtop Microscope that Grows with Your Needs
BM42 | BC43 WF
Affordable Widefield Fluoresence
- Imaging Modes: 2
- Brightfield & Differential Phase Contrast
- Widefield (BM42 confocal n.a.)
BC43 CF
Fast, Easy and Compact Confocal
- Imaging Modes: 3
- Brightfield & Differential Phase Contrast
- Widefield & Confocal
BC43 SR
Small in Size, Super in Resolution
- Imaging Modes: 4
- Brightfield & Differential Phase Contrast
- Widefield, Confocal & Super Resolution
Webinars – Learning Centre
MULTI-SCALE IMAGING
CANCER BIOLOGY
NEUROSCIENCE
CELL BIOLOGY
The Compact, Flexible and Easy to Use Microscope You Can Trust
BC43 is a super-compact unit that is rich in features and benefits, making it the ideal microscope for early-stage researchers and experienced microscopists alike. With no requirement for a darkroom, researchers can access a range of fluorescence microscopy techniques on the same bench as their sample preparation. This, coupled with the fast-learning curve, allows individual users and entire labs to become much more productive, without compromising results.
Optical performance is backed up by Andor’s new IQ / OQ quality control programme, which ensures that all BC43 microscopes operate within tightly controlled parameters, providing confidence that your data and experiments are reproducible in the future.
«We found the Andor benchtop confocal incredibly easy to set up and use. It produces great images and has become a real workhorse in our lab, handling different specimens from live zebrafish larvae to tissue sections and organoids.”
Prof. Dr. med. Ruxandra Bachmann-Gagescu | Molecular Life Science/University of Zurich
Fluorescence immunohistochemistry analysis of the retinal structure in a 10-day-old zebrafish larva. Nuclei labeled with DAPI in blue, opsins are represented in green and photoreceptor outer segments are delineated in magenta.
Picture credited by Department of Molecular Life Science | University of Zurich
Fluorescence immunohistochemistry examination of the mature zebrafish retina. Nuclei are depicted with DAPI staining (blue), acetylated tubulin is visualized in green and photoreceptor outer segments are highlighted in magenta.
Picture credited by Department of Molecular Life Science | University of Zurich
Stitched image of an intact larval brain, displaying nuclear DNA labeling in blue, acetylated tubulin immunostaining in green and primary ciliary are highlighted in magenta.
Picture credited by Department of Molecular Life Science | University of Zurich
Fluorescence immunohistochemistry of iPSC-derived cortical neurons at 70 days of differentiation.
Nuclei are shown with DAPI staining (blue), MAP2 is stained green, TUBB3 is stained magenta and B4-Spectrin is stained white.
Picture credited by Department of Molecular Life Science | University of Zurich
Widefield Fluorescence, Confocal, and Super Resolution Microscopy
The BC43 benchtop microscope portfolio utilises imaging technology from the award-winning Dragonfly confocal system, and provides fast and remarkably flexible imaging. From routine imaging to advanced super resolution, BC43 provides researchers with the ultimate productivity hack, delivering stunning images in seconds, hassle-free.
Widefield Fluorescence Microscopy
BC43 WF and all other models support widefield fluorescence microscopy. The most widely used fluorescence imaging technique by researchers. Typically used for imaging tissue sections and cultured cells (fixed or living).
Widefield is sometimes preferred over confocal and super resolution for having the highest sensitivity and detecting lower sample signals. It also offers the fastest imaging speeds to capture dynamic processes in live cells. Adding ClearView™ digital confocal removes blur making simple 3D imaging possible.
Confocal Microscopy
BC43 CF, upgradable from the BC43 WF model, includes our patented spinning-disk confocal technology, providing fast and remarkably flexible imaging. No expertise needed to achieve routine confocal imaging. Capture superb images at least 10 times faster than point scanner-based systems!
BC43 is a prime solution for 3D imaging of thick samples. A high sensitivity, high-speed imaging camera makes it perfect for live-cell confocal imaging.
Super Resolution Microscopy
ncluded in BC43 SR, upgradeable for all other models, our super-resolution module powers through the diffraction limit of even the highest quality optics to ~140nm.
This mode can be used in combination with either widefield fluorescence or confocal delivering the highest detail for intracellular structures like actin filaments and microtubules for clarity on network structure, vesicles aiding multi-label identification, and other targets like muscle fibre structure.
A two-dimensional method which can still be used in 3D structures to gain deeper insight.
Transmitted Light Microscopy
All models deliver two different transmitted light options for amazing imaging flexibility:
- Use Brightfield Mode to visualise the development of larger organisms whose structures naturally deliver high contrast images
- For thinner samples and/or those with higher transparency, use Differential Phase Contrast (DPC) mode to draw out their structure.
You can also mix and match modes like DPC and Widefield for even more imaging flexibility!
TOMM20 ALEXA 488 (green), alpha Tubulin ALEXA 555 (yellow) and SPY650 Actin (red)
Picture credited by Nicolas Schilling – Light & Electron Microscopy Center for Microscopy and Image Analysis | UZH
Powerful and Intuitive Acquisition Software
At the heart of BC43 is a software that is not only feature-rich and flexible to your needs, it is easy-to-use as well:
- Imaris rendering engine – stunning real-time 3D images
- Clear and concise user-interface – acquire images in no time
- Multi-dimensional imaging protocols – easy 3D time lapse or multi-position montage
- Upgradable – add new features when you need them
Comprehensive Image Analysis Software with AI Tools
All Andor Benchtop Microscopes come with the introductory Imaris Quantify package for users to:
-
- Visualise their 2D/3D/4D images in the world’s leading interactive microscopy image analysis software
- Identify and characterize objects within their images via traditional intensity-based and AI methods
- Report counts, morphology metrics, intensity values, and many more statistics to find differences across images and experiments
- Create high-resolution snapshots and multi-dimensional movies with ease
Additional Imaris modules, are also available including tools for tracking and reporting motility metrics, quantifying cells and their intracellular organelles, and tracing neurons for reporting their specific characteristics.
Feedback from BC-43 workshop
“I have to admit, I was impressed with the BC43 system. It’s incredibly user-friendly and the speed at which it acquires images for the 3D projection is great! Not to mention, the image quality was also good. If the BC43 system becomes available at our facility, I would absolutely love to use it. I can see how it would make our work so much easier and more efficient.”
Drosophila Embryos
Pictures credited by Biozentrum, University of Basel
Drosophila
Pictures credited by Biozentrum, University of Basel
Easy Fluorescence Microscopy Adapting to Your Research
Andor’s benchtop microscope range has been developed in collaboration with leading biologists; for use across life science applications and is designed to be part of a single room workflow. Our benchtop microscopes will bring instant clarity and depth to your research, whatever your sample, in ways previously only found in larger, more complex and expensive imaging systems.
Cancer
BC43 addresses the broad scales and types of imaging protocols and analysis related to cancer research. From super-resolved subcellular and cellular fixed and live cell experiments to large tissue samples and organoids requiring deeper imaging.
- Image monolayer and 3D cell cultures
- Follow cell dynamics (intercellular behaviour, migration and division)
- High productivity imaging of tissue preparations
- Analyse movement, cell lineage, count cells, features and more
- IQ-OQ quality control ensures ongoing confidence in your results.
Cell Biology
periments demand: from a simple 2D snapshot of a cell to understandinBC43 conquers the diverse range of protocols that Cell Biology exg dyn
BC43 conquers the diverse range of protocols that cell biology experiments demand: from a simple 2D snapshot of a cell, even for super-resolution, to understanding dynamic processes with time-lapse.
BC43’s unrivalled clarity and speed will save you time without compromising resolution (X,Y or Z) or field of view.
- Confocal mode for fast, high-resolution imaging of tissues
- Widefield mode for higher sensitivity and maximum speeds
- ClearView™ technology boosts image clarity in all models.
Core Facilities
Andor’s benchtop microscope is a compact, but fully featured system for routine imaging. It can be the imaging workhorse you are looking for, freeing up your sophisticated systems with advanced tools for those who need them.
Simplicity of use has been designed into BC43 to reduce training time and ongoing support of users, enabling labs and facilities to become more efficient. Listening to microscopy community groups like QUAREP-LiMi our IQ-OQ programme confirms reliable performance at all times.
Researchers will rapidly become competent in acquiring excellent 3D images, and higher multi-dimensional experiments on their own.
- Four excitation lines (405, 488, 561 & 640nm)
- Up to 5 imaging objectives (exchangeable)
- Multiple imaging modes offers flexibility whatever the sample and whatever your budget and needs.
Developmental Biology
Cut through the challenges of your 3D world and capture images in perfect clarity – from the very first rounds of cell division to the fully developed organism.
Extract crisp 2D images or explore stunning 3D volumes in a fraction of the time you’re used to. Andor’s Benchtop Confocal delivers fast, high-resolution imaging of developing model organisms such as zebrafish and Drosophila.
- Choose a model appropriate to your sample needs
- Purchase or upgrade to confocal for thick specimens/models
- Boost productivity 10-fold compared with traditional confocals
- No sacrifices to resolution, field of view or 3D detail for speed.
Neuroscience
Break through the burden of countless images demanded by your neuroscience research with a ten-fold speed boost, compared to conventional confocal microscopes.
Flexible imaging modes cover sensitive live imaging of neuronal processes to large scale imaging of entire neuronal networks in 3D.
- Select BC43 WF for tissue sections and cultures. BC43 CF for cleared tissue, expansion, organoids and more
- Outstanding real-time 3D visualisation for immediate insight
- Super-resolution available for high detail investigation
- IQ-OQ assurance on image accuracy
- Dedicated image analysis tools with Imaris: automated process tracing, auto depth, spine detection and more.
Tissue Imaging
Capture stunning 2D and 3D images from live tissue models to whole cleared-tissue preparations.
BC43 is a true workhorse instrument for large sample imaging, capturing in a tenth of the time of conventional confocal systems and imaging deeper than widefield epifluorescence with deconvolution or digital clearing.
- Time-saving, large-view, high-resolution imaging of tissue
- Capture huge samples with tiled volume imaging and stitching
- Innovative analysis tools available with Imaris: machine learning object classification, spatial analysis, volume and morphology and many more.
Specifications
| Microscope Unit - Imaging Modes |
|
| Microscope Unit - Imaging Methods |
Single colour, multicolour, z-stacking (volume), time-lapse, multi-position, multi-well, montage and 2/3D stitching |
| Microscope Unit - ClearView GPU |
Clears image of non-specific sample background signal and improves resolution beyond the normal optical limits |
| Camera - Resolution |
6.5 um pixel; 2048 x 2000 pixels (4.1MP) |
| Camera - QE2 |
Up to 82% |
| Camera - Field of view (mm) |
18.4mm (diagonal) |
| Camera - Cooling |
0°C |
| Camera - Images |
16-bit, monochrome |
| Illumination - Fluorescence |
4 fixed wavelengths of
|
| Illumination - Transmitted light |
Broad spectrum visible light LED |
| Optics - Objective Lens Nosepiece |
Motorised 5 position turret |
| Optics - Objective Magnifications |
BC43 is supplied with 2x objective for sample overview. Select additional supported objective lenses from 10x to 60x magnification |
| Optics - Precision motorised x,y stage |
Travel Range = 100mm x 80mm Resolution = 10nm |
| Optics - Z-Control & Focus |
Range = 14.5mm |
| Optics - Autofocus "Seek & Lock" Technology |
Sample “Seek & Lock”. Finds focal plane for new sample and maintains focus stability during time-lapse experiments. |
| Optics - Sample Vessels Supported |
Optimal imaging through glass, for imaging through plastic vessels, consult your sales representative |
| Optics - Incubation (option) |
Stage-top incubator. Sliding lid for easy sample access and exchange. Objective heater for oil-immersion objectives. |
| Workstation |
|