Scale Your Science. Confocal Power for the Big Data Era.
Description
Exceptional Performance, Certified Quality, High Productivity
The Andor BC43 Benchtop Microscope is your versatile platform for advanced imaging. It delivers stunning high-quality images across multiple modalities, supporting 2D/3D, widefield, and confocal acquisitions.
For applications demanding the highest detail, the super-resolution option offers true performance down to 140 nm - achieved easily with the push of a button.
Key Advantages:
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Certified Quality: Our certification guarantees the reproducibility and reliability of your data, ensuring consistency between experiments and research groups.
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Intuitive Workflow: The BC43 includes the powerful Fusion Benchtop software for straightforward multimodal imaging acquisition.
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Market-Leading Analysis: Seamlessly integrated with Imaris, the market leader in 3D/4D image analysis, giving you the tools to extract meaningful insights from your data.
Small in Size, Big in Performance.
Fast, Compact, Upgradable, Premium Microscopy
With certified quality, BC43 offers productivity and image excellence while maintaining the ease of use of a conventional entry-level widefield system, delivering high-end features at a competitive price.
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Advanced Imaging Technology – Sharp 2D & 3D images instantly
- Enhanced visualisation software – Intuitive and powerful
- Easy to use 3D joystick
- Light-tight lid – No requirement for a darkroom
- Optimal performance for thick samples and live cell dynamics
- Flexible – Confocal, Fluorescence and Transmitted Light modes
- New Super-resolution Mode – push past the diffraction limit to reveal the inner workings of the cell
- Analysis Workflows – Imaris image analysis adding meaning to your images
Key Features
- Compact – fits on a bench, no need for a dark room, built-in antivibration mechanism.
- Super-fast learning curve – new users can be independent within 30 min to 2 h – minimal training and user support needed.
- Target productivity – dual microlens spinning disk integrated with a sensitive sCMOS camera and specialised SW modules.
- Modular and upgradable – Widefield or Confocal, in-field upgrade , Modular software to adapt to user needs.
- Unique & certified quality – IQ/OQ quality control – Certified by experts in the scientific imaging field QUAREP-LiMi.
- Live cell imaging – dual microlens spinning disk, efficient light collection results in gentle imaging, (image model organisms for days).
- Versatility – with 4 lasers, 4 imaging modalities and a range of objectives, all your important microscopy lab experiments can be done hassle-free.
- Image Analysis – Includes Imaris the gold standard software that delivers image analysis with precision, speed and user-friendly workflows.
Confocal, Widefield & Transmitted Light Microscopy All-In-One
The BC43 benchtop microscope is offered in 2 models: BC43 Widefield (BC43 WF) and BC43 confocal (BC43 CF). Both models include transmitted light options, DPC and brightfield, and can be upgraded to super resolution.
The BC43 widefield is:
- An excellent solution for imaging thinner samples (either cells or tissues)
- Field upgradable to the confocal model (easy upgrade if your needs grow)
- Included in the IQ/OQ program
The BC43 confocal delivers:
- Outstanding images through the use of technology from our award-winning Dragonfly confocal
- Exceptional results in either thin and thick tissue samples (eg organoids)
- Fast, flexible and high-quality 3D images
- Hassle-free routine imaging from brightfield to confocal
- Certified quality due to our innovative IQ/OQ program
Confocal Microscopy
Speed, Productivity, Image excellence and Quality
With BC43 confocal, the researchers can:
- Capture superb confocal images at least 10 times faster than point scanner-based systems
- Image deep into thick samples in 3D easily.
- Image live samples for days without phototoxicity or photobleaching.
- Image fast live events due to a high-sensitivity, high-speed imaging camera.
- Take advantage of the motorised XYZ stage to increase productivity, either by acquiring multiple points or large organism montages, all in one experiment
Widefield Fluorescence Microscopy
Image excellence, Certified quality, Field upgradable
BC43 widefield system:
- Ideal for imaging light-sensitive and thin samples
- Offers the fastest imaging speeds to capture dynamic processes in live cells.
- Detects lower sample signals than in confocal mode.
- Boosts productivity by the use of: 1) extremely precise XYZ stage, and 2) the power of fusion software.
- With Clearview™ GPU deconvolution removes sample blur and enhances resolution.
If user a needs grow and a powerful confocal system is required to image deeper, and with increased resolution, the BC43 widefield can be field upgraded to the BC43 confocal.
Transmitted Light Microscopy
Label-free imaging, Enhanced contrast, no Phototoxicity
BC43 delivers two different transmitted light options for excellent imaging flexibility:
Brightfield Mode to visualise the development of larger organisms, whose structures naturally deliver high-contrast images.
Differential Phase Contrast (DPC) is ideal for transparent samples, allowing the visualisation of structural detail.
Advantages of BC43´s transmitted light methods:
- No need for sample labelling
- Low energy illumination: no phototoxicity, no photobleaching
- Reveal full organism boundaries – visualise the spatial context.
- Overlay with widefield or confocal: Integrate protein localisation into the organism’s environment
Super Resolution Microscopy
Fast, One click results
We have developed and optimised our popular SRRF-Stream+ technology for exceptional ease of use. Visualise cell structure, protein interactions, membrane fusion, signal transduction events and more.
SRRF-Stream+ is compatible with:
- Standard labelling protocols.
- Thick and thin samples.
- Widefield and confocal imaging modalities.
- Live imaging protocols.
Overcome the diffraction limit by a factor of 1.5x to 1.7 x down to ~140nm.
«We found the Andor benchtop confocal incredibly easy to set up and use. It produces great images and has become a real workhorse in our lab, handling different specimens from live zebrafish larvae to tissue sections and organoids.”
Prof. Dr. med. Ruxandra Bachmann-Gagescu | Molecular Life Science/University of Zurich
Fluorescence immunohistochemistry analysis of the retinal structure in a 10-day-old zebrafish larva. Nuclei labeled with DAPI in blue, opsins are represented in green and photoreceptor outer segments are delineated in magenta.
Picture credited by Department of Molecular Life Science | University of Zurich
Fluorescence immunohistochemistry examination of the mature zebrafish retina. Nuclei are depicted with DAPI staining (blue), acetylated tubulin is visualized in green and photoreceptor outer segments are highlighted in magenta.
Picture credited by Department of Molecular Life Science | University of Zurich
Stitched image of an intact larval brain, displaying nuclear DNA labeling in blue, acetylated tubulin immunostaining in green and primary ciliary are highlighted in magenta.
Picture credited by Department of Molecular Life Science | University of Zurich
Fluorescence immunohistochemistry of iPSC-derived cortical neurons at 70 days of differentiation.
Nuclei are shown with DAPI staining (blue), MAP2 is stained green, TUBB3 is stained magenta and B4-Spectrin is stained white.
Picture credited by Department of Molecular Life Science | University of Zurich
TOMM20 ALEXA 488 (green), alpha Tubulin ALEXA 555 (yellow) and SPY650 Actin (red)
Picture credited by Nicolas Schilling – Light & Electron Microscopy Center for Microscopy and Image Analysis | UZH
Fusion Benchtop – Powerful and Intuitive Acquisition Software
User-Friendly. Easy to Learn. Built to Deliver.
At the heart of BC43 is a software that is easy to use yet feature rich, flexible and modular, allowing the system to grow with your research needs. Fusion Benchtop includes features that enhance productivity and provide high-quality image visualisation.
Some of the fusion bench top features include:
- Multi-dimensional imaging protocols – 3D time lapse or multi-position montage
- Real-time 3D images – visualise data in 3D while acquiring (using the Imaris rendering engine)
- Deconvolution – activated on the protocol – no need for post-processing
- Stitching – activated on the protocol – Stitch Montage acquisitions in XYZ
- Spiral montage – effortless sample overview
- Irregular montage – acquire only the relevant montage tiles
- Focus Map – correct for tilt and sample wobbling
- Z intensity correction – visualise all the details deep inside your sample
Imaris – Market leader in Image Analysis
Comprehensive Image Analysis Software with AI Tools
All Andor Benchtop Microscopes come with the Imaris Quant package for users to:
- Visualise their 2D/3D/4D images in the world’s leading interactive microscopy image analysis software.
- Identify and characterize objects within their images via traditional intensity-based and AI methods.
- Report counts, morphology metrics, intensity values, and many more statistics to find differences across images and experiments.
- Create high-resolution snapshots and multi-dimensional movies with ease.
Additional Imaris modules are also available including tools for tracking and reporting motility metrics, quantifying cells and their intracellular organelles, and tracing neurons for reporting their specific characteristics.
Feedback from BC-43 workshop
“I have to admit, I was impressed with the BC43 system. It’s incredibly user-friendly and the speed at which it acquires images for the 3D projection is great! Not to mention, the image quality was also good. If the BC43 system becomes available at our facility, I would absolutely love to use it. I can see how it would make our work so much easier and more efficient.”
Drosophila Embryos
Pictures credited by Biozentrum, University of Basel
Drosophila
Pictures credited by Biozentrum, University of Basel