qPCRBIO Probe 1-Step Virus Detect – Witec AG

High Sensitivity

Developed with a high concentration 4x mix format, qPCRBIO Probe 1-Step Virus Detect offers greater sensitivity and flexibility by allowing more sample to be added to each reaction, and for smaller reaction volumes to be used with confidence. This is particularly beneficial in high throughput assays or for clinical diagnostic testing where accurate and reproducible detection of low copy viral templates is of crucial importance. This specialised kit allows accurate and sensitive detection of viral sequences over a broad range of input RNA, down to 4 copies tested per reaction (0.8 copies per μL).

Robust and reproducible RT-qPCR

qPCRBIO Probe 1-Step Virus Detect uses
UltraScript Reverse Transcriptase for fast and
efficient cDNA synthesis up to 55ºC. The PCR step
is powered by PCRBIO HS Taq DNA Polymerase,
which employs antibody-mediated hot start
technology for specific amplification of virusderived
cDNA, with improved tolerance to the
common PCR inhibitors found in clinical samples.

Universal detection kit

qPCRBIO Probe 1-Step Virus Detect contains all
the components needed for rapid and accurate
RT-qPCR, requiring only the addition of primers,
probes, template and water. The kit is compatible
with all qPCR instruments and a wide range
of probe technologies, including TaqMan®,
Scorpions® and molecular beacon probes.

Applications

• RNA virus detection
• Diagnostic real-time PCR
• High throughput assays
• TaqMan®, Scorpions® and molecular beacon probes
• COVID-19 / SARS-CoV-2 diagnostics and research