Protein A Resin - 5mL | 50% slurry – Witec AG

GenScript Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.

Protein A Resin can also be used for immunoprecipitation of proteins, protein complexes or antigens.

The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

The Resin is reusable up to 10 times with no significant loss of binding capacity.

Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus , binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens.

Note: High temperature heating is not recommended. The agarose melts above 65°C.