Anti-His Affinity Resin

Art No | L00439

Anti-His Affinity Resin

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from 560.00 CHF

GenScript Anti-His Affinity Resin is designed for purification of His-tagged protein.

Description

It is composed of agarose and conjugated THETM anti-His monoclonal antibody.

His-tagged protein in the crude samples can bind to the anti-His monoclonal antibodies coupled with the agarose, and the nonspecific proteins can be eliminated by washing. As a result, the His-tagged protein will finally be eluted with high recovery. Moreover, this product can be used for immunoprecipitation of His-tagged protein.

Note:

The product is designed for purification and immunoprecipitation of His-tagged protein, especially for the sample failed by Ni-NTA Agarose Chromatography purification.

Selection Guide Resins (PDF)

Supplier

Genscript
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Specifications

Beads structure	4% Agarose
Beads size	45-165 μm, averagely 90 μm
Coupled antibody	THE^TM His Tag Antibody, mAb, Mouse (Cat.No A00186)
Binding capacity	More than 0.6 mg of His-tagged protein per mL settled resin
Specificity	N-terminal/C-terminal/internal/4 x His/5 x His/6 x His-tagged protein
Stability	Stable at 2-8 °C for 1 year
Storage	2-8 °C. Avoid freezing the resin
Repeat usage	Generally the resin can be recycled at least 4 times. If maintained properly, the resin may be recycled 10 times with minor binding capability los.
Elution methods	Acid, alkaline and peptide competitive elutions
Reagents compatibility	Compatible with mostly used reagents at certain concentrations
Constistency	Lot-to-lot constitency proved by strict production procedure