AmMag™ Ni Magnetic beads 10mg 6xHis-tagged protein (27 kD) – Witec AG

GenScript's AmMag^TM Ni Magnetic Beads are optimized for quick and efficient purification of polyhistidine-tagged proteins under native as well as denaturing conditions.

The beads use a nickel-charged TED (tris-carboxymethyl ethylene diamine) ligand to purify His-tagged proteins. The beads are resistant to chelators such as EDTA and DTT, and can be regenerated using 1M NaOH.

• Quick and convenient separation accomplished by magnetic force
• High binding capacity with 10mg of His-tagged protein/ml settled beads
• The AmMag Ni magnetic beads can be regenerated and reused up to 100 times in absence of chelators
• Extremely low nonspecific binding

Application

Purification of His-tagged proteins

These superparamagnetic beads are about 70 μm in diameter and their strong magnetic properties allow them to attach very fast to the magnet. The AmMag Ni-charged beads are chelator resistant (up to 100mM EDTA and 20mM DTT). These magnetic beads enable the purification to be performed even with a very high concentration of chelator in the sample, but in such situation, the re-use of beads is impacted. The beads are supplied as 25% slurry in 20% ethanol.