Protein G MagBeads >10mg goat IgG

Art No | L00274

Protein G MagBeads >10mg goat IgG

180.00 CHF

Brochure

Size: 2mL (25% slurry)

GenScript Protein A, G and A/G MagBeads are developed for quick, convenient and reproducible immunoprecipitation(IP) and small scale purification of immunoglobulin.

Description

GenScript Protein G MagBeads are superparamagnetic beads covalently bound with recombinant protein G for antibody purification.

The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Protein G MagBeads have a binding capacity of more than 10 mg Goat IgG per 1 ml settled beads.

Key Features

Quick and convenient separation accomplished by magnetic forces
High binding capacity more than 10 mg goat IgG/ml settled beads
Extremely low nonspecific binding
Can be reused > 30 cycles*

Application

Purification of antibodies from small & large cell cultures

Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native Protein G has three IgG binding domains and also the sites for albumin and cell-surface binding. Albumin and cell-surface binding domains have been eliminated from recombinant Protein G to reduce nonspecific binding. Protein G has greater affinity than Protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike Protein A, Protein G does not bind to human IgM, IgD and IgA.

*CIP was performed after each cycle, by incubating with 5 CV 0.1 M NaOH for 1 hr. Then magnetic beads were rinsed with excessive dd water and re-equilibrated with binding buffer before next purification cycle.

Storage: store at 4°C - do not freeze

Product manual (PDF)

Magnetic Beads Selection Guide

GenScript Protein A, G and A/G MagBeads are developed for quick, convenient and reproducible immunoprecipitation(IP) and small scale purification of immunoglobulin. By covalently pre-coupling recombinant Protein A, G and A/G to the surface of superparamagnetic beads, these MagBeads provide users simple, easy and time-saving procedures for immunomagnetic separation.

Due to the elimination of non-IgG binding domains and other unknown function fragments, the recombinant Protein A, G and A/G both display high affinity for subclasses of IgG from many species including human, rabbit and mouse etc. This permits the purification of IgGs from cell culture supernatants, serum and ascites or other starting samples. In addition, Protein A, G and A/G MagBeads can also be used to immunoprecipitate target proteins from crude samples using a selected primary antibody. To prevent co-elution of the antibody, it is necessary to chemically cross-link the antibody to protein A, G and A/G coated beads prior to the immunoprecipitation steps.

By applying magnetic separation technology, the Protein A, G and A/G MagBeads supply researchers with fast, easy and efficient IP/isolation procedures.

A magnetic separation rack is also needed to use the MagBeads. All steps take place in a single tube, without the need for columns, centrifugation, or pre-treatment. Extremely gentle magnetic handling prevents loss of your target IgGs or protein and keeps them intact throughout the procedure.

Protein A, G, and G/G MagBeads are the perfect tools for immunoprecipitation and small-scale IgG purification.

Principle
The sample containing antibody is added to the Protein A MagBeads. The antibody will bind to beads during a short incubation. Then the beads‐bound antibody can be eluted off from the beads using a magnetic separation rack.

Magnetic separation eliminates the changes of micro tubes, minimizes the loss of sample and removes excessive steps of traditional centrifugation method.

For Expression screening

MagBeads Advantages:

Simpliﬁed handling of samples without the need for a chromatographic system
Ability to analyze several samples simultaneously thus reducing the total time required for analysis
Minimum sample loss and higher recovery than protein A resin

Case study 1:

For 5 mL volume, AmMag protein A magnetic beads compared with prepacked resin column

MagBeads: 500 μL AmMag protein A magnetic beads

Prepacked column: 1 mL prepacked Monoﬁnity A resin (combined with AKTA)

Samples containing human IgG1 produced in CHO cells which were continuously taken from a bioreactor during a two-week cell culture period.

Case study 2:

For 50 mL volume, AmMag protein A magnetic beads compared with pre-packed resin column

MagBeads: 500 μL AmMag protein A magnetic beads

Prepacked column: 0.6 mL Robocolumn A Resin (combined with Tecan)

Samples containing human IgG1 produced in CHO cells which were continuously taken out from day 14 cell culture period.

Time: Using MagBeads, 5 samples were puriﬁed in 60 minutes. Using column method, 5 samples were puriﬁed in 4 hours. MagBeads method saves 75% time.

Purity: Samples 1-5 were puriﬁed with MagBeads, sample 6 was puriﬁed by column puriﬁcation.

Using MagBeads method, 1 sample was puriﬁed in 1.5 hours. Using column method, 1 sample was puriﬁed in 3 hours.

MagBeads method saves 50% of time.

For large scale protein purification

AmMag protein A magnetic beads characteristics:

Simpliﬁed handling of samples without the need for a chromatographic system
No clarifying steps needed
Scalability: Simple capture of antibodies from large sample volumes (microliter to liliter scale)

Case study 3:

1 L antibody expression cell culture media (100 mg antibodies per L)

MagBeads: 5 mL AmMag protein A magnetic beads

Prepacked column: 5 mL Monofnity A Resin (combined with AKTA)

Samples containing human IgG1 produced in CHO cells which were continuously taken out from day 14 cell culture period

Supplier

Genscript
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Accessories

Art No | L00722

AmMag™ MR-mini magnetic rack for 2mL tubes

GenScript provides magnetic racks that help you purify proteins from small scale (≤ 2 mL), midi-scale (2 – 15 mL) and large-scale (15- 50 mL) cell cultures and lysates. These modular magnetic racks allow fast protein purification while simplifying the procedure.

240.00 CHF