MonoRab™ Anti-DYKDDDDK Magnetic Beads - 1mL

Art No | L00835-1

MonoRab™ Anti-DYKDDDDK Magnetic Beads - 1mL

520.00 CHF

Size: 1mL

GenScript MonoRabTM Anti-DYKDDDDK Magnetic Beads are developed and optimized for protein purification.

Description

GenScript MonoRab^TM Anti-DYKDDDDK Magnetic Beads are super paramagnetic beads with diameter of 70 μm, covalently coated with a rabbit monoclonal anti-DYKDDDDK antibody.

The beads are supplied as 50% slurry in TBS with 50% glycerol and 0.02% sodium azide. The MonoRab^TM Anti-DYKDDDDK Magnetic Beads have a binding capacity of 1.5 mg DYKDDDDK-tagged protein (Size: 50KDa) per 1 ml settled beads. The beads can be re-used for at least 5 times.

Key Features

Effecient: Quick, easy and convenient magnetic separation procedures
High capacity: Each ml beads can bind 1.5 mg DYKDDDDK-tagged protein(50KDa)
Specific: Based on our MonoRab™ rabbit monoclonal antibody technology
Roubust: Can tolerate high salt concentration up to 2 M NaCl and to buffers with pH as low as 2.5
Can be re-used for at least 5 times

Based on our new rabbit monoclonal antibody technology (MonoRab^TM), this beads offers high binding capacity, high purity, and withstand stringent washing steps, especially with buffers containing high concentration of salt. The beads are resistant to up to 2 M NaCl, and to buffers with pH as low as 2.5.

Storage: store at -20°C

Product manual (PDF)

Principle

The sample containing DYKDDDDK fusion proteins is added to the MonoRabTM Anti-DYKDDDDK Magnetic Beads and incubated on a shaker for a short time for binding. The DYKDDDDK fusion proteins bound to the beads can be eluted by using alkaline elution buffer, competitive elution buffer or PAGE gel sample buffer.

Magnetic separation eliminates the need for pre-cleaning of the sample by centrifugation, minimizes the loss of sample, and makes the process more user friendly.

Supplier

Genscript
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Specifications

Matrix	Super paramagnetic beads
Average bead size	70 μm
Ligand	MonoRab™ monoclonal antibody against DYKDDDDK tag
Binding capacity	> 1.5 mg protein (50KDa)/ml beads
Regeneration	≥5 times
Storage solution	50% slurry in TBS with 50% glycerol and 0.02% sodium azide
Storage temperature	-20°C

Accessories

Art No | L00722

AmMag™ MR-mini magnetic rack for 2mL tubes

GenScript provides magnetic racks that help you purify proteins from small scale (≤ 2 mL), midi-scale (2 – 15 mL) and large-scale (15- 50 mL) cell cultures and lysates. These modular magnetic racks allow fast protein purification while simplifying the procedure.

240.00 CHF