Size: 4mL (25% slurry)
GenScript Protein A, G and A/G MagBeads are developed for quick, convenient and reproducible immunoprecipitation(IP) and small scale purification of immunoglobulin.
Description
GenScript Protein A MagBeads MX are super paramagnetic beads of average 50 μm in diameter, covalently coated with recombinant Protein A.
The beads are supplied as 25% slurry in 20% ethanol. The Protein A MagBeads MX have a binding capacity of more than 30 mg human IgG per 1 ml settled beads.
Key Features
- Quick and convenient separation accomplished by magnetic means
- High binding capacity more than 30 mg human IgG /ml settled beads
- Extremely low nonspecific binding
- Optimized homogeneous recombinant ligands
- Can be reused > 30 cycles*
Application
Purification of antibodies from small & large cell cultures
Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs, mainly through Fc regions. Native Protein A has five IgG binding domains and many unknown-function repeated sequences. Recombinant Protein A only contains five high-affinity IgG binding domains to reduce nonspecific binding.
*CIP was performed after each cycle, by incubating with 5 CV 0.1 M NaOH for 1 hr. Then magnetic beads were rinsed with excessive dd water and re-equilibrated with binding buffer before next purification cycle.
Storage: store at 4°C - do not freeze
GenScript Protein A, G and A/G MagBeads are developed for quick, convenient and reproducible immunoprecipitation(IP) and small scale purification of immunoglobulin. By covalently pre-coupling recombinant Protein A, G and A/G to the surface of superparamagnetic beads, these MagBeads provide users simple, easy and time-saving procedures for immunomagnetic separation.
Due to the elimination of non-IgG binding domains and other unknown function fragments, the recombinant Protein A, G and A/G both display high affinity for subclasses of IgG from many species including human, rabbit and mouse etc. This permits the purification of IgGs from cell culture supernatants, serum and ascites or other starting samples. In addition, Protein A, G and A/G MagBeads can also be used to immunoprecipitate target proteins from crude samples using a selected primary antibody. To prevent co-elution of the antibody, it is necessary to chemically cross-link the antibody to protein A, G and A/G coated beads prior to the immunoprecipitation steps.
By applying magnetic separation technology, the Protein A, G and A/G MagBeads supply researchers with fast, easy and efficient IP/isolation procedures.
A magnetic separation rack is also needed to use the MagBeads. All steps take place in a single tube, without the need for columns, centrifugation, or pre-treatment. Extremely gentle magnetic handling prevents loss of your target IgGs or protein and keeps them intact throughout the procedure.
Protein A, G, and G/G MagBeads are the perfect tools for immunoprecipitation and small-scale IgG purification.
Principle
The sample containing antibody is added to the Protein A MagBeads. The antibody will bind to beads during a short incubation. Then the beads‐bound antibody can be eluted off from the beads using a magnetic separation rack.
Magnetic separation eliminates the changes of micro tubes, minimizes the loss of sample and removes excessive steps of traditional centrifugation method.
For Expression screening
MagBeads Advantages:
- Simplified handling of samples without the need for a chromatographic system
- Ability to analyze several samples simultaneously thus reducing the total time required for analysis
- Minimum sample loss and higher recovery than protein A resin
Case study 1:
For 5 mL volume, AmMag protein A magnetic beads compared with prepacked resin column
MagBeads: 500 μL AmMag protein A magnetic beads
Prepacked column: 1 mL prepacked Monofinity A resin (combined with AKTA)
Samples containing human IgG1 produced in CHO cells which were continuously taken from a bioreactor during a two-week cell culture period.
Case study 2:
For 50 mL volume, AmMag protein A magnetic beads compared with pre-packed resin column
MagBeads: 500 μL AmMag protein A magnetic beads
Prepacked column: 0.6 mL Robocolumn A Resin (combined with Tecan)
Samples containing human IgG1 produced in CHO cells which were continuously taken out from day 14 cell culture period.
Time: Using MagBeads, 5 samples were purified in 60 minutes. Using column method, 5 samples were purified in 4 hours. MagBeads method saves 75% time.
Purity: Samples 1-5 were purified with MagBeads, sample 6 was purified by column purification.
Using MagBeads method, 1 sample was purified in 1.5 hours. Using column method, 1 sample was purified in 3 hours.
MagBeads method saves 50% of time.
For large scale protein purification
AmMag protein A magnetic beads characteristics:
- Simplified handling of samples without the need for a chromatographic system
- No clarifying steps needed
- Scalability: Simple capture of antibodies from large sample volumes (microliter to liliter scale)
Case study 3:
1 L antibody expression cell culture media (100 mg antibodies per L)
MagBeads: 5 mL AmMag protein A magnetic beads
Prepacked column: 5 mL Monofnity A Resin (combined with AKTA)
Samples containing human IgG1 produced in CHO cells which were continuously taken out from day 14 cell culture period
Accessories
GenScript provides magnetic racks that help you purify proteins from small scale (≤ 2 mL), midi-scale (2 – 15 mL) and large-scale (15- 50 mL) cell cultures and lysates. These modular magnetic racks allow fast protein purification while simplifying the procedure.
GenScript provides magnetic racks that help you purify proteins from small scale (≤ 2 mL), midi-scale (2 – 15 mL) and large-scale (15- 50 mL) cell cultures and lysates. These modular magnetic racks allow fast protein purification while simplifying the procedure.