PCRBIO Ultra Mix Red - Long range and "difficult" PCR

Art No | PB10.33

PCRBIO Ultra Mix Red - Long range and "difficult" PCR

Choose size / variation:

from 75.00 CHF

Size: 2 x 1mL | 80 Reactions

PCRBIO Ultra Mix Red has been engineered for the amplification of extremely difficult templates, with the convenience of direct loading onto agarose gels without the need for additional loading buffer.

Description

The latest polymerase developments are combined with antibody-mediated hot start technology to deliver outstanding performance for all your PCR applications.

Increased PCR success rates with difficult templates
Exhibits high tolerance to PCR inhibitors
Efficient specific amplification from complex templates including GC-rich sequences (up to 80%)
Antibody-mediated hot start for unrivalled detection of low copy number templates
Advanced buffer chemistry including Mg and dNTPs
High yields under standard and fast PCR conditions
3 fold higher fidelity than Taq
Available as a convenient 2x ready mix with the option of a red dye for direct gel loading

PCRBIO Ultra Mix Red is powered by PCRBIO Ultra Polymerase, a highly robust enzyme designed for efficient and reliable amplification of challenging and complex targets, even under difficult conditions such as the presence of inhibitors. The enzyme and buffer system have been developed to give superior PCR performance and higher success rates on a broad range of templates, including complex genomic DNA and targets with a high GC content.

Our antibody-mediated hot start formulation prevents the formation of primer dimers and non-specific products, allowing for specific and sensitive amplification from low copy number target sequences.

PCRBIO Ultra Mix Red has an error rate of approximately 1 error per 5.0 x 105 nucleotides incorporated. PCR products are A-tailed and may be cloned into TA cloning vectors.

Applications

Difficult PCR - GC and AT-rich DNA
Low copy template detection
Crude sample PCR
Colony PCR
Long range PCR
Multiplex PCR
TA cloning
Next generation sequencing

Storage

On arrival, products should be stored between -30 and -15°C. If stored correctly the kit will retain full activity for 12 months.

Material Safety Data Sheet (PDF)

Product manual (PDF)

FAQs

Figure 1. Long PCR products visualised on agarose gel

Amplification of 0.8kb, 1.0kb, 1.8kb, and 2.7kb fragments of the GAPDH gene, a 4.6kb fragment of the RBL15 gene, and a 5.9kb fragment of the MYH6 gene. The starting template amount is 20ng (50ng for the 5.9kb fragment) of mouse genomic DNA and is diluted 2 to 5 fold. PCRBIO Ultra Polymerase amplifies the range of fragment lengths indicated with high yield and specificity.

Figure 2. GC rich products visualised on agarose gel

Amplification of 0.5kb (P1) and 0.6kb (P2) fragments of the ATXN2 gene with GC contents of 69% and 71%, respectively, using 20ng of mouse genomic DNA
as template and a range of annealing temperatures from 67°C to 60°C (B-E). PCRBIO Ultra Polymerase efficiently amplifies GC rich templates >65% GC and is recommended for templates up to 80% GC.