PCRBIO Taq Mix Red - Routine Application PCR

Art No | PB10.13

PCRBIO Taq Mix Red - Routine Application PCR

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from 98.00 CHF

PCRBIO Taq Mix Red uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. The enzyme and buffer system allow for superior PCR performance on complex templates such as mammalian genomic DNA.

Description

PCRBIO Taq Mix Red is powered by PCRBIO Taq DNA Polymerase - a robust enzyme for all your everyday PCR applications including genotyping, screening and library construction. PCRBIO Taq Mix Red is room temperature stable for 4 weeks and has the added convenience of a preloaded red dye suitable for direct loading and tracking during agarose gel electrophoresis.

Red mix for direct loading onto agarose gels
Increased PCR success rates with amplicons up to 6kb
Ultra low background DNA
Advanced buffer chemistry including Mg and dNTPs
High yields under standard and fast PCR conditions
Efficient specific amplification from complex templates including GC rich and AT rich sequences
Stable at 25°C and 37°C for 4 weeks

PCRBIO Taq DNA Polymerase performs consistently well on a broad range of templates (including both GC and AT rich). The enzyme has 5’-3’ exonuclease activities with the same error rate as wild-type taq DNA polymerase, approximately 1 error per 2.0 x 105 nucleotides incorporated. PCRBIO Taq DNA Polymerase production uses an enhanced 12 step purification strategy which includes physical, chemical and enzymatic removal of host DNA. PCR products are A-tailed and may be cloned into TA cloning vectors.

PCRBIO Taq Mix Red provides the research community with a convenient, affordable and
versatile routine application master mix that allows you to amplify with the highest speed, yield, specificity and consistency on the market.

Applications

Routine application PCR
TA cloning
High throughput PCR
Methylated DNA
Crude sample PCR
Standard and fast PCR
Efficient specific amplification from GC and AT-rich sequences

Storage

On arrival, products should be stored between -30 and -15°C. If stored correctly the kit will retain full activity for 12 months.

Material Safety Data Sheet (PDF)

Product manual (PDF)

FAQs

Figure 1.

Shows amplification of a 1.2kb fragment of 60% GC GAPDH, from human genomic DNA, in a 3 fold dilution from left to right.

The starting concentration is 200ng of DNA and is diluted to 0.7pg in the 7th dilution. PCRBIO Taq DNA Polymerase (row 1) is able to amplify lower concentration template DNA compared with competitor P and I (rows 2 and 3).

Figures 2.

Shows no change in activity is detected in PCRBIO Taq DNA Polymerase after 28 days at 4˚C, 25˚C and 37˚C.

Figure 3.

Shows amplification of the same 1.2kb fragment of 60% GC GAPDH in a 3 fold dilution from left to right as in figure 1.

Fast cycling conditions are used of 5 secs denaturation and 30 secs annealing/extension.

Under fast conditions PCRBIO Taq DNA Polymerase (row 1) is able to amplify lower concentration template DNA compared with competitor P and I (rows 2 and 3).

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