PCRBIO HS Taq Mix - Novel hot-start technology

Art No | PB10.22

PCRBIO HS Taq Mix - Novel hot-start technology

Choose size / variation:

from 170.00 CHF

PCRBIO HS Taq DNA Polymerase is an advanced antibody-mediated hot start DNA polymerase designed for fast, highly specific and sensitive PCR.

Description

Whether you need a hot start assay for high-throughput, automated reaction setup or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.

Hot start technology for unrivalled detection of low copy number templates
Increased PCR success rates with amplicons up to 6kb
Ultra-low background DNA
Advanced buffer chemistry including Mg and dNTP
Efficient specific amplification from GC and AT-rich sequences
High performance under both fast and standard cycling conditions
Gives increased PCR throughput with existing thermal cyclers
Stable at 25°C and 37°C for 4 weeks

PCRBIO HS Taq DNA Polymerase is an advanced antibody-mediated hot start enzyme engineered for fast, highly specific and sensitive PCR. The latest developments in polymerase technology and buffer chemistry enhance PCR speed, yield and specificity, giving a consistently high performance on a broad range of templates including both GC and AT-rich sequences.

Proprietary antibodies inhibit polymerase activity until an initial activation step at 95°C, preventing the formation of primer dimers and non-specific products, giving improved specificity and sensitivity compared to other methods. The enzyme and buffer system allow for superior PCR performance on complex templates such as mammalian genomic DNA. Whether you need a hot start assay for high-throughput, automated reaction setup or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.

PCRBIO HS Taq DNA Polymerase is also available as a convenient, ready-to-use 2x mix containing all reaction components except primers and template. PCRBIO HS Taq Mix Red contains a preloaded red dye for direct gel loading.

Applications

Genotyping
High-throughput PCR
Standard and fast PCR
Routine and multiplex PCR
TA cloning
Inhibitor tolerant PCR direct from bacterial culture, blood and urine
Colony PCR
‘Difficult’ PCR - GC and AT-rich DNA

Storage

On arrival, products should be stored between -30 and -15°C. If stored correctly the kit will retain full activity for 12 months.

Material Safety Data Sheet (PDF)

Product manual (PDF)

FAQs

Figure 1.
Shows the amplification of a 1kb fragment of Beta-Actin under standard cycling conditions.

Primer extension is prevented during reaction set up and first temperature ramp. Primer dimer amplification diverts DNA polymerase activity from the amplicon of interest and reduces sensitivity in the assay. The top row is PCRBIO HS Taq DNA Polymerase and the 2nd row is an equivalent product from Kapa Biosystems.

Figure 2.

Shows no change in activity is detected in PCRBIO HS Taq DNA Polymerase after 28 days at 4˚C, 25˚C and 37˚C.

Figure 3.
Shows amplification of a 400bp gene under fast cycling conditions from human genomic DNA.

40 cycles of 5 seconds denaturation at 95˚C and 5 seconds annealing/extension at 60˚C. A 10 fold dilution series of template starting from 100ng was used. The top row is PCRBIO HS Taq DNA Polymerase, the 2nd row is the equivalent product from Kapa Biosystems and the 3rd row is the equivalent product from Invitrogen.

Available sizes: