PCRBIO Taq DNA Polymerase - Robust Application PCR (incl. dNTPs)

Art No | PB10.11

PCRBIO Taq DNA Polymerase - Robust Application PCR (incl. dNTPs)

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from 110.00 CHF

PCRBIO Taq DNA Polymerase is an affordable, versatile and robust enzyme for all your everyday PCR applications including genotyping, screening and library construction.

Description

An enhanced 12-step purification strategy together with an optimised buffer system enable PCRBIO Taq DNA Polymerase to amplify with the highest speed, yield and specificity.

Increased PCR success rates with amplicons up to 6kb
Ultra low background DNA
Advanced buffer chemistry including Mg and dNTPs
High yields under standard and fast PCR conditions
Efficient specific amplification from complex templates including GC and AT-rich sequences
Stable at 25°C and 37°C for 4 weeks

PCRBIO Taq DNA Polymerase is a robust enzyme for all your everyday PCR applications including genotyping, screening and library construction. PCRBIO Taq DNA Polymerase performs consistently well on a broad range of templates including both GC and AT rich.

PCRBIO Taq DNA Polymerase has 5’-3’ exonuclease activities, but no 3’-5’ exonuclease (proofreading) activity. The enzyme has the same error rate as wild-type taq DNA polymerase, approximately 1 error per 2.0 x 105 nucleotides incorporated.

PCRBIO Taq DNA Polymerase provides the research community with an affordable routine application polymerase that performs to the highest possible standard, with a versatility that allows you to amplify with the highest speed, yield, specificity and consistency on the market. PCR products generated are A-tailed and may be cloned into TA cloning vectors. The enzyme is room temperature stable for 4 weeks and is produced using an enhanced 12 step purification strategy which includes physical, chemical and enzymatic removal of host DNA.

For added convenience, PCRBIO Taq DNA Polymerase is available as a ready-to-use 2x mix containing all reaction components except primers and template. PCRBIO Taq Mix Red contains a red dye suitable for direct loading and tracking during agarose gel electrophoresis.

Applications

Routine application PCR
TA cloning
High throughput PCR
Methylated DNA
Crude sample PCR
Standard and fast PCR
Efficient specific amplification from GC and AT-rich sequences

Storage

On arrival, products should be stored between -30 and -15°C. If stored correctly the kit will retain full activity for 12 months.

Material Safety Data Sheet (PDF)

Product manual (PDF)

FAQs

Figure 1.

Shows amplification of a 1.2kb fragment of 60% GC GAPDH, from human genomic DNA, in a 3 fold dilution from left to right.

The starting concentration is 200ng of DNA and is diluted to 0.7pg in the 7th dilution. PCRBIO Taq DNA Polymerase (row 1) is able to amplify lower concentration template DNA compared with competitor P and I (rows 2 and 3).

Figures 2.

Shows no change in activity is detected in PCRBIO Taq DNA Polymerase after 28 days at 4˚C, 25˚C and 37˚C.

Figure 3.

Shows amplification of the same 1.2kb fragment of 60% GC GAPDH in a 3 fold dilution from left to right as in figure 1.

Fast cycling conditions are used of 5 secs denaturation and 30 secs annealing/extension.

Under fast conditions PCRBIO Taq DNA Polymerase (row 1) is able to amplify lower concentration template DNA compared with competitor P and I (rows 2 and 3).