IsoFast™ Hot Start Bst Polymerase

Art No | PB80.4015% OFF the entire range of PCR Biosystems Kits

IsoFast™ Hot Start Bst Polymerase

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from 136.00 CHF

15% OFF the entire range of PCR Biosystems PCR & qPCR Kits! Expires 31/12/2025

Amplify Smarter. Amplify Faster.

Description

Bst DNA polymerase combined with AptaLock™ reversible hot-start technology power the IsoFast® Hot Start Bst reagents. These products are ideal for all isothermal amplification workflows, giving more sensitive and reliable results faster than ever before.

IsoFast® Hot Start Bst Polymerase is a recombinant version of the large fragment of Geobacillus stearothermophilus (formerly known as Bacillus stearothermophilus, Bst) DNA Polymerase. It is engineered to retain 5’-3’ polymerase activity, but lacks 5’-3’ exonuclease activity. The enzyme’s strong strand displacement capability eliminates the need for denaturation required by standard PCR. Combined with powerful hot start technology, to minimise primer dimer formation and non-specific target amplification, this novel formulation is ideal for non-PCR based amplification.

AptaLock™ hot start for ultra-sensitive detection of DNA targets
Rapid polymerisation for faster time to results (as little as 10 mins)
Detect down to 3 target copies per μL
Ideal for both cold and room temperature setup
Improved speed and sensitivity for early target detection
High activity at a broad range of temperatures from 55-70
Available with and without fluorescent dye, and as a 2x ready mix

Applications

Whole genome amplification
Multiple displacement amplification
Rolling circle amplification
Isothermal amplification
Loop mediated isothermal amplification (LAMP)
Molecular diagnostics
Point-of-care testing

Hot start isothermal amplification

Hot start is important in isothermal amplification techniques because it helps to prevent non-specific amplification and false-positive results. Isothermal amplification methods, such as loop-mediated isothermal amplification (LAMP), rely on specific primers and enzymes to amplify a target sequence at a constant temperature, typically between 60°C and 65°C. These techniques are widely used in various applications, including molecular diagnostics and pathogen detection.

Why is hot start crucial in isothermal amplification?

Preventing non-specific amplification in Bst polymerase isothermal reactions: In isothermal amplification, it’s essential to ensure that the amplification process only targets the specific sequence of interest. Non-specific amplification can occur when primers bind to unintended regions of the template or when amplification enzymes prematurely initiate amplification. Hot start techniques involve inhibiting the amplification reaction until the reaction reaches the desired temperature. This prevents the formation of non-specific products during the initial stages of the reaction when the temperature is still below the optimal amplification temperature.
Enhancing Bst polymerase isothermal amplification specificity: By preventing premature amplification, hot start methods increase the specificity of the isothermal amplification reaction. This means that the reaction is less likely to amplify non-target sequences, reducing the risk of false-positive results.
Improving isothermal amplification sensitivity with Bst polymerase: Hot start techniques can also improve the sensitivity of isothermal amplification assays. By minimising non-specific amplification, more of the available reagents are reserved for amplifying the target sequence, increasing the efficiency of the reaction and the detection limit.

Material Safety Data Sheet (PDF)

Product manual (PDF)

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