IsoFast™ Hot Start Bst Polymerase

Art No | PB80.40

IsoFast™ Hot Start Bst Polymerase

Choose size / variation:

from 160.00 CHF

Ideal for all isothermal amplification workflows, giving more sensitive and reliable results faster than ever before.

Description

IsoFast™ Hot Start Bst Polymerase & Mix are the most recent and improved development of isothermal amplification technology, designed to deliver unparalleled specificity and speed in DNA amplification processes and designed to meet the demands of molecular diagnostics and research.

AptaLock™ hot start for ultra-sensitive detection of DNA targets
Rapid polymerisation for faster time to results (as little as 10 mins)
Detect down to 3 target copies per μL
Ideal for both cold and room temperature setup
Improved speed and sensitivity for early target detection
High activity at a broad range of temperatures from 55-70°C

Whether you are working with loop-mediated isothermal amplification (LAMP) or other isothermal amplification methods, IsoFast™ Hot Start Bst Polymerase 2x Mix guarantees exceptional sensitivity and reliability. This reagents harnesses the power of a hot start Bst DNA polymerase enzyme, which ensures minimal background amplification and maximises target specificity. With IsoFast™ Hot Start, your isothermal amplification experiments will be faster, more efficient, and easier than ever, setting new standards for molecular biology techniques in both diagnostic applications and research endeavours.

Hot start is important in isothermal amplification techniques because it helps to prevent non-specific amplification and false-positive results. Isothermal amplification methods, such as loop-mediated isothermal amplification (LAMP), rely on specific primers and enzymes to amplify a target sequence at a constant temperature, typically between 60°C and 65°C. These techniques are widely used in various applications, including molecular diagnostics and pathogen detection.

Material Safety Data Sheet (PDF)

Product manual (PDF)

Fig-1.-IsoFast-Hot-Start-vs-IsoFast-Bst-Mix-M13-time-to-result

Improved speed with IsoFast™ Hot Start Bst Polymerase

Isothermal amplification of the M13 bacteriophage target sequence in the scaffolding protein gene from the M13 bacteriophage genome using IsoFast™ Hot Start Bst Mix and IsoFast™ Bst Mix. A primer mix consisting of 0.2 µM for F3 and B3 primers, 1.6 µM for FIP and BIP primers and 0.8 µM for LoopF and LoopB primers was used. The total reaction volume was 25 µL. 8 serial dilutions of M13 ssDNA genome were used, starting with a stock of 0.5 ng/µL and using a dilution factor of 10, corresponding to the number of genome copies indicated in the plot. The reaction was run at 65 °C for 100 minutes. A BioRad CFX96 Touch instrument was used to record fluorescence every 10 seconds. The time to threshold indicates the time required to reach the same fluorescent threshold.

IsoFast™ Hot Start Bst Mix shows faster amplification when compared to IsoFast™ Bst Mix.

Fig-2.-IsoFast-Hot-Start-vs-NEB-WarmStart-mix-M13-time-to-result

Faster detection with both cold and ambient setup

Isothermal amplification of a target sequence in the scaffolding protein gene from the M13 bacteriophage genome using IsoFast™ Hot Start Bst Mix and NEB WarmStart LAMP Kit. A primer mix consisting of 0.2 µM for F3 and B3 primers, 1.6 µM for FIP and BIP primers and 0.8 µM for LoopF and LoopB primers was used. The total reaction volume was 25 µL. 8 serial dilutions of M13 ssDNA genome were used, starting with a stock of 0.5 ng/µL and using a dilution factor of 10, corresponding to the number of genome copies indicated in the plot. Reaction master mixes and plates were prepared either using cold blocks (cold setup) or at room temperature (ambient setup), for approximately 20 min. The reaction was run at 65 °C for 100 min. A BioRad CFX96 Touch instrument was used to record fluorescence every 10 seconds. The time to threshold indicates the time required to reach the same fluorescent threshold.

IsoFast™ Hot Start Bst Mix shows faster amplification when compared to NEB WarmStart LAMP Kit, both under cold and ambient setup.