IsoFast™ Bst 1-Step Mix – Witec AG

Applications

• Multiple displacement amplification
• Isothermal amplification
• Reverse Transcription Loop mediated isothermal amplification (RT-LAMP)
• Molecular diagnostics
• Field diagnostics

IsoFast® Bst 1-Step Mix is designed for reverse transcription of target RNA and subsequent isothermal amplification in a single tube.

Dual enzyme system

The kit utilises IsoFast® Bst Polymerase for its strong strand displacement activity, enabling DNA synthesis at a constant temperature (65˚C) without the need for thermal cycling. Representing the large fragment of Geobacillus stearothermophilus (formerly Bacillus stearothermophilus) DNA Polymerase, this portion of the protein catalyses the 5’-3’ synthesis of DNA but does not contain the 5’-3’ exonuclease domain.1 The enzyme is provided in a convenient 2x mix format.

Reverse transcription of target RNA is carried out by the thermostable and extremely active RTase Go, which is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase.

Rapid and consistent results

Designed for fast amplification, IsoFast® Bst 1-Step Mix gives rapid and consistent results across a wide range of sample types and is validated for qualitative detection of SARS-CoV-2 nucleic acid (Fig 1 and 2). The buffer chemistry ensures high yield and performance even under difficult conditions, for example when inhibitors are present.

IsoFast® Bst 1-Step Mix contains all the components for fast and sensitive isothermal amplification of RNA targets, requiring only the addition of primers, template and water. The kit is provided with a separate tube of fluorescent dye to allow real-time detection with any qPCR instrument.

This product is not suitable for PCR.