IsoFast™ Bst Mix

Art No | PB80.12

IsoFast™ Bst Mix

Size:

from 290.00 CHF

Amplify Faster. Detect Sooner. Discover More.

Description

Achieve unparalleled speed and sensitivity in your isothermal amplification workflows with the IsoFast® Bst Mix from PCR Biosystems. This advanced 2x mix is engineered to deliver rapid, robust, and reliable results for a wide range of DNA and RNA targets, including applications in molecular diagnostics, whole genome amplification, and field-based testing.

IsoFast® Bst Mix is a recombinant form of the large fragment of Bst DNA polymerase, containing 5′-3′ polymerase activity and strand displacement activity.

The enzyme offers fast amplification and strong strand displacement capabilities, making it ideal for nucleic acid amplification methods such as whole genome amplification, multiple displacement amplification and isothermal amplification.

Has strand-displacing 5′-3′ polymerase activity
Lacks 5′-3′ exonuclease activity
DNA synthesis is performed at a constant temperature
Operates over a broad temperature range, with an optimum of 65ºC
Gives rapid and consistent amplification across a wide range of templates
Supplied with an advanced 2-part buffer system for higher yields under difficult conditions
30 minute protocol
Flexible formats with and without fluorescent dye
Also available as a 2x mix and as a dual enzyme 1-step system for RNA amplification
Glycerol-free enzyme

Applications

Whole genome amplification
Multiple displacement amplification
Isothermal amplification
Loop mediated isothermal amplification (LAMP)
Molecular diagnostics
Field diagnostics

Accelerated Amplification: A unique, strand-displacing Bst polymerase enables DNA synthesis at a constant temperature (around 65°C), eliminating the need for a thermal cycler and dramatically reducing time to result.
Superior Sensitivity: Optimized for the detection of even low-copy number targets, giving you confidence in your data.
Convenient 2x Mix Format: Streamlines your workflow, reduces pipetting steps, and minimizes the risk of error, making it ideal for high-throughput applications.
Versatile Performance: Delivers consistent, high-yield amplification across a wide range of templates and sample types, even in the presence of inhibitors.
Optimized for Flexibility: Available with an optional fluorescent dye for real-time detection, allowing you to use any standard qPCR instrument.

Material Safety Data Sheet (PDF)

Product manual (PDF)

FAQs

PCRBIO Tm Calculator

Isothermal Amplification

Isothermal amplification refers to a range of methods that rely on an alternative approach to amplification that is not limited by the requirement for thermal cycling.

Instead of denaturing DNA by using high temperatures, isothermal amplification takes advantage of DNA polymerases that have high strand displacement capabilities, for example Bst or Phi29 DNA polymerases. These enzymes have the ability to ‘unzip’ DNA as they synthesise complementary strands, removing the need for thermocycler equipment as the reaction is carried out at a one temperature. Isothermal methods making use of strand displacing DNA polymerases include multiple displacement amplification, whole genome amplification and loop mediated isothermal amplification or LAMP.

PCR Biosystems’ IsoFast™ Bst range of products is based on the large fragment of Bst DNA polymerase and offers rapid and sensitive amplification of DNA and RNA targets at a constant temperature of around 65°C.