Collagenase NB 1 GMP Grade 2000 PZ U/Vial

Art No | N0002937

Collagenase NB 1 GMP Grade 2000 PZ U/Vial

5'210.00 CHF

Brochure

Size: ≥ 2000 DMC

For Pancreatic Cell Isolation and other GMP Applications

Description

Collagenase NB 1 GMP Grade is a highly purified collagenase grade.

This product is used for isolation of sensitive cells and guarantees high yields and viability.

In combination with Neutral Protease NB GMP Grade, Collagenase NB 1 GMP Grade is especially applicable to isolation of pancreatic islets of Langerhans. Collagenase NB 1 GMP Grade is recommended for applications like tissue engineering and transplantation into humans which have to meet GMP requirements.

Supplier

Nordmark Pharma GmbH
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Specifications

Origin	Clostridium histolyticum
Composition	Collagenase class l and class ll¹ ¹ Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
Molecular weight	70 – 120 kDa (collagenases)
Special features	Manufactured according to intl. GMP guidelines
Microbiological examination	Endotoxin^: Sterility (Ph. Eur.) Absence of Clostridia TAMC (Total Aerobic Microbial Count) ≤ 10/Vial TYMC (Total Yeasts/ Moulds Count) ≤ 10/Vial Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
Activities	Collagenase ≥ 2000 U/Vial (PZ^ activity according to Wünsch, 25 °C) Collagenase ≥ 3.000 U/mg lyophilisate (PZ^activity according to Wünsch, 25 °C) Neutral Protease ≤ 0.05 U/mg lyophilisate (DMC assay^*) ^*PZ Unit (acc. to Wünsch²): 1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1. ² Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler’s Z. Physiol. Chem. 333, 149-51*
Storage conditions	+2 °C to +8 °C
Shipping conditions	Cooled on blue or dry ice depending on the destination
Intended use	For clinical applications