GenScript Protein G Resin FF is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.
Description
- The recombinant protein G ligand is coupled to 4% highly cross-linked agarose.
- The static binding capacity of Protein G Resin FF is greater than 20 mg human IgG/ml settled resin.
- The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
- Average particle size: 90 μm (45-165 μm)
Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics.
Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.
Specifications
Ligand |
Recombinant Streptococcal protein G lacking the albumin-binding sites expressed in E. coli |
Number of IgG binding sites per ligand |
3 |
M.W. of ligand |
approx. 22 kDa |
PI of ligand |
4.69 |
Degree of substitution |
approx. 2mg protein G/mL settled resin |
Static binding capacity |
≥ 20 mg human IgG/ml settled resin |
Matrix spherical |
4% highly cross-linked Agarose |
Average particle size |
90 μm (45-165 μm) |
Recommended flow rate |
50-300cm/h |
Storage solution |
20% Ethanol |
Storage and Stability |
18 months when stored unopened at 2-8°C |