High Affinity Ni-Charged Resin FF Prepacked Column

Art No | L00683

High Affinity Ni-Charged Resin FF Prepacked Column

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from 94.00 CHF

GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins.

Description

High Affinity Ni-Charged Resin FF has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification.

This makes High Affinity Ni-Charged Resin FF Prepacked Column the excellent choice for high performance purification of polyhistidine-tagged proteins.

Compatible reagents:

Denaturants	Detergents	Reducing agents	Salts	Others
6 M Gu·HCl	2% Triton X-100	20 mM β-ME	4 M MgCl₂	50% glycerol
8 M Urea	2% Tween 20	1 mM DTT	5 mM CaCl₂	20% ethanol
	1% CHAPS		2 M NaCl	1 mM EDTA

Column compatibility

The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).

Selection Guide Resins (PDF)

Supplier

Genscript
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Specifications

Binding capacity	>40 mg of histidine-tagged protein /mL settled resin
Matrix spherical	6% highly cross-linked Agarose
Average particle size	90 μm (45-165 μm)
Storage solution	20% Ethanol
Storage & Stability	18 months when stored unopened at 2-8°C