PCRBIO Rapid Extract PCR Kit

Art No | PB10.24

PCRBIO Rapid Extract PCR Kit

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from 100.00 CHF

Eliminate the need for laborious and time-consuming DNA extraction methods with this simple, integrated extraction and amplification kit powered by the latest advances in hot start polymerase technology.

Description

PCRBIO Rapid Extract PCR Kit combines rapid DNA extraction with fast, highly specific DNA amplification in a convenient, easy-to-use format. Generate PCR-ready DNA in as little as 15 minutes, without any need for spin columns or hazardous chemicals.

Rapid, convenient, single-tube DNA extraction
Produces high yield, PCR-ready DNA in as little as 15 minutes
Maximised PCR speed, yield and specificity
Powered by PCRBIO HS Taq Mix Red for direct gel loading – saving you even more time
Ideal for complex templates
Suitable for multiple sample types including mouse tail clip and ear punch, animal tissue, hair follicle, buccal swab, mammalian blood and FFPE tissue

PCRBIO Rapid Extract PCR Kit has been developed for fast, efficient amplification of DNA from a variety of tissues and is particularly suited to solid tissue such as mouse tail or mouse ear. Sample processing is simplified and contamination risks minimised as DNA extraction is performed in a single tube, removing the need for multiple washing steps.

Extracted DNA is amplified in a proprietary buffer system using PCRBIO HS Taq Mix Red. Our antibody-mediated hot start polymerase uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and sensitivity. Primer dimer formation and non-specific amplification are prevented giving superior specificity ideal for complex templates such as mammalian genomic DNA. The mix contains a red dye suitable for direct gel loading without the need for additional loading buffer.

Applications

Genotyping
Transgene detection
Knockout analysis
Sequencing

Storage

On arrival, products should be stored between -30 and -15°C. If stored correctly the kit will retain full activity for 12 months.

Material Safety Data Sheet (PDF)

Product manual (PDF)

FAQs

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PCRBIO Rapid Extract PCR Kit can be used to generate PCR-ready DNA in as little as 15 minutes, without the need for spin columns or multiple washing steps.

a) PCRBIO Rapid Extract PCR Kit was used to extract DNA from 3mg of mouse tail clipping following the standard 15 minute protocol.

The extraction was repeated using equivalent extraction kits from alternative manufacturers. A serial three-fold dilution series was made from each supernatant. PCRBIO HS Taq Mix Red was used to amplify a 1kb fragment of mouse GAPDH gene from each dilution. Results were compared by agarose gel electrophoresis.

Row 1 shows results from PCR Biosystems, row 2 from Kapa Biosystems, row 3 from Bioline, row 4 from Sigma and row 5 from Fermentas.

b) PCRBIO Rapid Extract PCR Kit was used to extract DNA from 3mg of mouse ear clipping following the standard 15 minute protocol.

The extraction was repeated using equivalent extraction kits from alternative manufacturers. A serial two fold dilution series was made from each supernatant. The supplied polymerase was used to amplify a 2.5kb fragment of the mouse Calnexin gene from each dilution. Results were compared by agarose gel electrophoresis.

Row 1 shows results from PCR Biosystems, row 2 from Kapa Biosystems, row 3 from Bioline and row 4 from Sigma.

Supplier

PCR Biosystems
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Specifications

5x PCRBIO Rapid Extract Buffer A	80 Reactions: 1 x 1.6mL 400 Reaction: 5 x 1.6mL
10x PCRBIO Rapid Extract Buffer B	80 Reactions: 1 x 800μL 400 Reactions: 5 x 800μL
2x PCRBIO HS Taq Mix Red	80 Reactions: 2 x 1mL 400 Reactions: 10 x 1mL
Reaction Volume	50μL