ALTO 16-channel benchtop SPR system

ALTO - digital high‑throughput benchtop SPR system

The world’s first digital high‑throughput benchtop SPR system.

Description

Surface Plasmon Resonance Powered by Digital Microfluidics.

Alto is the world’s first SPR instrument to integrate digital microfluidics (DMF), artificial intelligence (AI), and nanotechnology to provide high quality, label-free interaction analysis. DMF technology enables all fluidics to be disposable, allowing for precise handling of 2uL sample volumes, crude sample compatibility and zero maintenance.

Alto’s compatibility with automation for both hardware and software provides
sample-in/answer-out data, industry leading throughput, and 24/7 runtime. Every aspect of Alto is designed with user-friendliness and accessibility in mind - from the flexible 16-channel design to the intuitive data analysis platform.

Alto is capable of supporting operation in GXP and 21 CFR Part 11 in compliance with regulatory demands.

Industry-Leading Data Quality
Minimize noise and dispersion; maximize sensitivity
The fastest rise times at <0.1s

High-Throughput Powered by Automation and AI
16 channels, intelligent assay design/optimization, automated serial dilutions
Fully compatible with plate & liquid-handling robots

Sample-Friendly
Only 2μL required for full kinetics
Analyze crude samples with ease

User-Friendly
Free of high-maintenance fluidic components and obsolete software

Alto is the world’s first SPR instrument to integrate digital microfluidics (DMF), artificial intelligence (AI), and nanotechnology.

DMF technology enables all fluidics to be disposable, allowing for precise handling of 2μL sample volumes, crude sample compatibility and zero maintenance. Alto’s compatibility with automation for both hardware and software provides sample-in/answer-out data, industry leading throughput, and 24/7 runtime.

Every aspect of Alto is designed with user-friendliness and accessibility in mind - from the flexible 16 channel design to the intuitive data analysis platform.

Applications:

Kinetics/Affinity Characterization
Kintetics/Affinity Screening
Competition Assays
Yes / No Binding
Concentration Analysis
Thermodynamics
Epitope Mapping/Binning

Compatible with:

Proteins
Antibodies
Nucleic Acids
Small Molecules
Carbohydrates
Lipids
Cells
Viruses
Nanoparticles

APPLICATION NOTES

Kinetic and epitope characterization of influenza antiviral targets using Alto™ digital surface plasmon resonance

Label-free Characterization of ImmTAC® Bispecifific
Molecules for T-Cell Redirecting Immunotherapy using Alto™

Label-Free Characterization of the Interleukin-6 Receptor and
Anti-IL-6R Antibody Drug Tocilizumab using Alto™ Digital SPR

Comparison of Single-Sequence and
Multi-Sequence Kinetic Analysis of a
Protein-Antibody Interaction on Alto

Protein interaction analysis using Alto™ digital surface plasmon resonance

Characterization of Biomolecular Interactions using Alto:
Comparison to Conventional SPR

Mass Transport Limited (MTL) Kinetics Analysis with Alto™ Digital SPR

What is Digital Microfluidics?

DMF is a liquid-handling technology capable of accurately controlling and manipulating discrete nanodroplets with electricity on a standard microwell plate.

Why DMF?

DMF fluidic handling technology removes the need for any physical pumps, valves, or tubes and replaces them with a low cost disposable cartridge, providing higher data quality and less hands-on time.

How does SPR work with DMF?

We’ve integrated our proprietary localized surface plasmon resonance (LSPR) sensors into DMF cartridges – creating 16 independently addressable channels on a single cartridge.

Advantages of using DMF-powered SPR

Decoupling of flow rate and sensor position from dispersion, increasing data quality

500X less sample volume required— get full kinetics from a 2μL sample volume

Decoupling of interaction time from sample volume, increasing assay flexibility

Automated serial dilutions—reducing human error and saving time

ALTO Cartridges

Alto Cartridges have completely revolutionized the interaction of fluidics and sensors for SPR.

A Cartridge is a well-plate sized consumable that integrates reagent/sample storage, fluid handling, and sensor surfaces. Samples, buffers and reagents are contained in wells on the cartridge, and dispensed towards the sensor in software-controlled nanodroplets.

Precise volumes and automated serially diluted concentrations interface with a fiber optic sensor tip, manufactured with the same quality and consistency (<2% CV) as our existing localized SPR sensors.

Surface Chemistries

Carboxyl for any amine group using EDC/NHS coupling
NTA for histidine tagged targets
Streptavidin for biotin tagged targets
Biotin for streptavidin coupled targets
GST for GST tagged targets
Protein A for IgG based antibodies
Amine for any carboxyl group using EDC/NHS coupling
Liposome Binding (LIP) for liposomes/membrane proteins
Hydrophobic (MEM) for lipid monolayer
Thiol for thiol or maleimide tagged targets
Gold for non-functionalized and for custom surface chemistry development and targets with thiol groups

Alto Software Suite

Alto features an end-to-end software suite that is powered by AI, built directly into the instrument, and leverages the power of the cloud. Flexible and intelligent assay design and optimization enables efficient planning of experiments. Serial dilutions are fully automated and controlled by software, reducing hands-on time and human error. With cloud connectivity, real-time data and wizards will clearly communicate experiment progress and can be accessed by any licensed device connected to the internet.

Features

Intuitive and easy-to-operate built in PC with customizability based on level of SPR experience
Guided setup, quality control and shutdown procedures
Software Wizards to guide experiment set-up, optimization and quality control
Full test automation with the ability to create, save and load test templates
Real-time 16-channel data of binding interactions featuring error warnings & experiment suggestions
Seamless and flexible transition of real-time data into data analysis software
Multiple instruments can be networked together and controlled easily from 1 interface

Supplier

Nicoya Lifesciences Inc.
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Specifications

Sample Volume	2μL
Rise Time	<0.1s
Association rate (kon)	<1e⁹ 1/(M*s)
Dissociation Rate (koff)	1e-⁶ to 1.0 (1/s)
Affinity Range (KD)	1e-¹² to 1e-⁴ (M)
Automation	Compatible with robots for 24/7 runtime
Compliance	GXP and 21 CFR Part 11* *Alto is capable of supporting operation in GXP and 21 CFR Part 11 in compliance with regulatory demands.
Operating Environment	15°C–35°C 20–80% RH Non-Condensing 0m–2000m Altitude
# Optical Channels	16 independently addressable
Reference : Standard	1:1 standard, any combination possible
Ligands	Up to 16
Analytes	Up to 64 Analytes/Cartridge
Dilutions	Automated Serial Dilutions
Crude Compatibility	Yes
Hands on Time	<30 mins
Temperature Control	8°C–50°C in sensor area 8°C in sample storage area
Instrument Size	35W x 50.5D x 40.4H (cm)
Instrument Weight	18kg