DNBSEQ-G400RS NGS Genetic Sequencer – Witec AG

Art No | 900-000170-00
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DNBSEQ-G400 is built with a new chip system that can flexibly support a variety of different sequencing modes.

Description

The DNBSEQ-G400 adopts a novel chip system that supports various modes of sequencing and is integrated with optimally designed optical and biochemical systems to execute the sequencing process within a relatively shorter time for a more streamlined sequencing experience for the user.

  • 2 different sized flow cells, FCL achieving 1800 million reads, and FCS achieving 550 million reads
  • 55 – 1440 GB per run
  • Only 48 hours to sequence a PE100 run at full capacity
  • Supporting a range of read lengths, including but not limited to SE400, PE100, PE150, PE200
  • Supporting sequencing and data analysis in a range of areas including scientific research, basic medicine, forensics, and agriculture

 

DNBSEQ-G400 PERFORMANCE PARAMETERS

READ LENGTHS DATA OUTPUT DATA QUALITY Q30* RUN TIME**
SE50-FCL 75-90G >85% ~16h
SE100-FCL 150-180G >85% ~28h
SE400-FCL 600-720G >70% ~109h
PE100-FCL 300-360G >85% ~54h
PE150-FCL 450-540G >75% ~78h
PE200-FCL 600-720G TBD TBD
SE100-FCS 55G >85% ~12-14h
PE100-FCS 110G >85% ~26h
PE150-FCS 165G >75% ~38h

*The percentage of base above Q30 is the average of an internal standard library over the entire run. The actual performance is affected by factors such as sample type, library quality, and insert fragment length.

**Run time was calculated based on dual-flow cell mode, and includes sample loading,sequencing, base calling and data processing.

 

DNBSEQ-G400 SPECIFICATIONS

MODEL DNBSEQ-G400
No. of Flow Cell/run 2
Type of Flow Cell FCS FCL
No. of lanes/Flow Cell 2 4
Max. reads/Flow Cell* 550M 1500-1800M
Read lenghts SE100
PE100
PE150
SE50
SE100
SE400
PE100
PE150
PE200

*The maximum number of effective reads are based on the sequencing of an internal standard library. Actual output may vary depending on sample type and library perparation method.

DNBSEQ-G400 NGS Genetic Sequencer: Versatility to satisfy multiple needs. Supports a range of read lengths, including SE50, SE100, SE400, PE100, PE150, PE200

DNBSEQ-G400 adopts an innovative “Flow Cell” system which can support various sequencing modes, and an optimised optical and biochemical system which enables the whole sequencing process to complete within a short period of time, offering the user a simplified and streamlined sequencing experience.

MGI’ S PROPRIETARY DNBSEQTM TECHNOLOGY

INCREASED ACCURACY
No PCR amplification required. Our unique Rolling Circle Replication (RCR) technology employed in DNBSEQTM library construction eliminates errors associated with PCR. Only the original template DNA is used to generate copies and therefore amplification errors do not accumulate, resulting in greater accuracy for detection of significant mutations such as lndels and SNPs.

DECREASED DUPLICATES
Optimized Patterned Array ensures that only one single DNB is attached at each spot, which results in greater saturation of DNB on the Flow Cell with unprecedented uniformity. This enables an industry-leading with reduced duplicate rate.

REDUCED INDEX HOPPING
MGI platform’s unique library preparation method and RCR amplification results in much lower index hopping rates compared with other platf orms, at a rate of 0.0001%~0.0004%.

DNB Technology
DNBSEQ-G400 NGS Genetic Sequencer: graphical user guide

Basic Scientific Research

  • Whole Genome Sequencing
  • Targeted Region Sequencing
  • Single Cell Sequencing
  • Epigenetic Sequencing
  • Whole Exome Sequencing
  • Non-coding RNA Sequencing
  • Transcriptome Sequencing
  • RNASeq

Data Analysis

  • Built-in basic analytical software completes the imaging analysis and base calling automatically and produces standard FastQ data files including sequences and base calling metrics
  • WGS Analysis System
  • WES Analysis System
  • PGS Analysis System
  • Cancer Hotspot Mutation Analysis System
  • Pathogen Fast Identification System

Supplier

MGI Tech Co. Ltd.
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Specifications

DNBSEQ-G400

for research only

No. of Flow Cell per run

2

Type of Flow Cell | No. of lanes | Max reads/Flow Cell
  • FCS: 2 lanes, 550M
  • FCL: 4 lanes, 1500-1800M
Dimensions | Power
  • LxWxH: 1086 x 756 x 710mm
  • Weight: 200kg
  • Power Type:100~240V,50/60Hz
  • Rated Power:1200VA
Control Computer Configurations
  • CPU:Intel Xeon E5 10Core x 2 2.2GHz
  • Internal Storage:256GB RAM
  • HDD:16TB
  • SSD:480G
  • Operating System:Windows 10 Enterprise
Operating Environment Requirements
  • Temperature:19-25℃
  • Relative Humidity:20% RH-80% RH,non-condensing
  • Atmospheric Pressure:70-106 kPa
  • Waterproof Rating:IPX0

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