Art No | MM1397-500
PAGE-MASTER Protein Standard Plus - 500 µl
84.00 CHF
Broad Multi Color Pre-Stained Protein Standard is a special-designed protein standard.
Description
The standard is a mixture of ten highly purified prestained proteins ranging from 5 kDa to 270 kDa, covalently coupled with four different chromophores. The 30 kDa band is red, 50 kDa band is green, 270 kDa band is orange, and other bands are blue. Broad Multi Color Pre-Stained Protein Standard is designed for observing protein separation during SDS-PAGE, verifying western transfer efficiency on membranes, and approximating the size of proteins.
Applications
- Monitoring protein migration during SDS-PAGE.
- Estimating protein sizes on SDS-PAGE gels.
- Verifying transfer efficiency on membranes for Western Blot.
- Locating a protein of interest for excision from an unstained preparative gel.
Specifications
| Storage Buffer |
62.5 mM Tris-H3PO4 (pH 7.5), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol |
| Storage |
Stable at -20°C for up to 24 months or 4°C for up to 3 months. Aliquots are recommended before use to avoid repeated freeze-thaw cycles |
| Procedures |
1. Thaw this product at room temperature. Do NOT boil protein marker. 2. Mix gently and thoroughly to ensure that the solution is homogeneous. 3. Bands intensity may vary according to gel type, thickness, and staining condition. Please adjust loading volume from 2.5 µl to 10 µ |
| Recommendations |
1. Completely thaw and mix this product at room temperature before using 2. This product is only suitable for roughly estimating the molecular weight of protein samples |
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