WB-MASTER Protein Standard - 250 µl – Witec AG

Art No | M00521
79.00 CHF
79.00 CHF

Description

WB-MASTER Protein Standard is designed for convenient protein identification in western blot. This standard consists of seven recombinant proteins with molecular weight of 20 kDa, 30 kDa, 40 kDa, 50 kDa, 60 kDa, 80 kDa and 120 kDa. Each of the proteins contains an IgG banding site that is able to bind to primary or secondary antibodies derived from a wide range of host species.

The standard thus enables direct visualization of both the protein marker and users' samples on the same western blot membrane without any additional reagents.

Supplier

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Specifications

Calculation

The apparent molecular weights of its seven protein bands are 20 kDa, 30 kDa, 40 kDa, 50 kDa, 60 kDa, 80 kDa and 120 kDa

Storage

Store at -20°C
Avoid repeated freezing and thawing

Applications

Approximating the size of proteins for Western blots

Notes
  • Do NOT boil this product.
  • For best results, the loading volume of the marker should be optimized for different experiment conditions (from 2.5 µl to 10 µl).
  • This marker binds to the constant region of IgG, so protein A or protein G fluorephore conjugates cannot be used as the secondary antibody.

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